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Team:TU Darmstadt/Protocols/Affinity chromatography
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# wash the column with dist. water | # wash the column with dist. water | ||
# load the column with 12 mL NiCl<sub>2</sub> solution | # load the column with 12 mL NiCl<sub>2</sub> solution | ||
| - | # wash the column with [ https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS buffer] | + | # wash the column with [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS buffer] |
Now the column is ready to use. | Now the column is ready to use. | ||
Revision as of 16:58, 16 September 2012
Affinity chromatography
Procedure
Column preparation
Before use the column needs to be activated:
- drain the ethanol
- wash the column with dist. water
- load the column with 12 mL NiCl2 solution
- wash the column with PBS buffer
Now the column is ready to use.
Columns can be reused. In oder to store them:
- wash the coumn with EDTA buffer
- discard the flowthrough and wash two times with 20% ethanol
- close up the column with Parafilm and store at 4°C
