Team:Buenos Aires/Results/Strains

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Revision as of 00:37, 16 September 2012

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- Strains characterization
- Strains description
- Fluorescence screening
- Screening of strain proportion
- Auxotrophy confirmation
- Coculture in liquid medium
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Strain characterization

a)	Description of strains,

Through our experiments we worked with the following strains:

H+T
Strain ID	Relevant Auxotrophies	Fluorescence	Used as
TCY 3043	(-H-T)	No fluorescence	Negative control
TCY 3190	(+H-T)	YFP + (Induced CFP)	For coculture
TCY 3265	(-H+T)	CFP	For coculture
TCY 3154
CFP +(induced YFP)	Positive Control

Table: Hystidine (H) and Tryptophane (T) auxotrophies per strain, type of fluorescence and description of most common utilization during the experiments.

Nearly 15 other similar strains were evaluated and discarded due to several reasons (low screening potentiality; requirement of hormones for fluorescence induction; high reverting rate of auxotrophies, among others)

@@ Line 5: / Line 5: @@
 == Strain characterization ==
-'''bold''', a)	Description of strains
+ '''a)	Description of strains,'''
 Through our experiments we worked with the following strains:
@@ Line 15: / Line 15: @@
 |-
 |TCY 3043
-|-H-T
+|(-H-T)
 |No fluorescence
 |Negative control
 |-
 |TCY 3190
-|+H-T
+|(+H-T)
 |YFP + (Induced CFP)
 |For coculture
 |-
 |TCY 3265
-|-H+T
+|(-H+T)
 |CFP
 |For coculture
@@ Line 36: / Line 36: @@
-'''bold''', Table: Hystidine (H) and Tryptophane (T) auxotrophies per strain, type of fluorescence and description of most common utilization during the experiments.
+ '''Table:''' Hystidine (H) and Tryptophane (T) auxotrophies per strain, type of fluorescence and description of most common utilization during the experiments.
 Nearly 15 other similar strains were evaluated and discarded due to several reasons (low screening potentiality; requirement of hormones for fluorescence induction; high reverting rate of auxotrophies, among others)