Team:Buenos Aires/DataPage
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Data Page
In order for the cross-feeding scheme to work we need each strain to export the amino acid they produce (either Histidine or Tryptophan). To achieve this we created a devices design to secrete to the medium an His (or Trp) rich peptides.
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Basic DNA structure of the devices with their constitutive parts |
- Kozak consensus sequence for initiation of translation.
- Signal peptide that targets the product of the gene for secretion.
- Trojan peptide to increase internalization in target cell.
- Payload: this is the exported amino acid rich domain of the protein.
The input of the devices are PoPS and the output is secreted amino acids, so the devices are PoPS -> exported AA transducers. In principle any PoPS generating part can be used.
You can read more about the design process here
We built 2 His-export devices, and 2 Trp-export devices:
- His-export I (<partinfo>BBa_K792009</partinfo>)
- Trp export I (<partinfo>BBa_K792010</partinfo>)
- His-export II (<partinfo>BBa_K792011</partinfo>)
- Trp export II (<partinfo>BBa_K792012</partinfo>)
To achive this, we had to create several new basic biobricks:
- Kozak sequence from yeast α-factor mating pheromone (MFα1) (<partinfo>BBa_K792001</partinfo>)
- Secretion tag from yeast α-factor mating pheromone (MFα1) (<partinfo>BBa_K792002</partinfo>)
- HIV TAT penetratin (<partinfo>BBa_K792003</partinfo>)
- Polyarginine trojan peptide (<partinfo>BBa_K792004</partinfo>)
- PolyHa, a Histidine rich peptide (His-Tag) (<partinfo>BBa_K792005</partinfo>)
- TrpZipper2, a Tryptophan rich peptide water soluble and monomeric (<partinfo>BBa_K792006</partinfo>)
- PolyHb, a stable Histidine rich peptide designed by us (<partinfo>BBa_K792007</partinfo>)
- PolyWb, a stable Tryptophan rich peptide designed by us(<partinfo>BBa_K792008</partinfo>)