Team:Buenos Aires/DataPage


Data Page


In order for the cross-feeding scheme to work we need each strain to export the amino acid they produce (either Histidine or Tryptophan). To achieve this we created a devices design to secrete to the medium an His (or Trp) rich peptides.

Crossfeeding-device-design v01.jpg
Basic DNA structure of the devices with their constitutive parts

  • Kozak consensus sequence for initiation of translation.
  • Signal peptide that targets the product of the gene for secretion.
  • Trojan peptide to increase internalization in target cell.
  • Payload: this is the exported amino acid rich domain of the protein.

The input of the devices are PoPS and the output is secreted amino acids, so the devices are PoPS -> exported AA transducers. In principle any PoPS generating part can be used.

You can read more about the design process here

We built 2 His-export devices, and 2 Trp-export devices:

To achive this, we had to create several new basic biobricks:

  • Kozak sequence from yeast α-factor mating pheromone (MFα1) (BBa_K792001)
  • Secretion tag from yeast α-factor mating pheromone (MFα1) (BBa_K792002)
  • HIV TAT penetratin (BBa_K792003)
  • Polyarginine trojan peptide (BBa_K792004)
  • PolyHa, a Histidine rich peptide (His-Tag) (BBa_K792005)
  • TrpZipper2, a Tryptophan rich peptide water soluble and monomeric (BBa_K792006)
  • PolyHb, a stable Histidine rich peptide designed by us (BBa_K792007)
  • PolyWb, a stable Tryptophan rich peptide designed by us(BBa_K792008)