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Team:Goettingen/week18-2
From 2012.igem.org
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| - | <h2><b> | + | <h2><b>V08_27 </b></h2><br> |
<b>Transformation of FlhDC-contructs into E.coli strain Mg </b><br> | <b>Transformation of FlhDC-contructs into E.coli strain Mg </b><br> | ||
<ul> | <ul> | ||
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| - | <h2><b> | + | <h2><b>V09_04 </b></h2><br> |
| - | <b>Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br> | + | <b>V09_04_1 Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | <li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | ||
</ul> | </ul> | ||
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<br> | <br> | ||
| - | + | <b>V09_04_2 Biobrick Standardization of motA, motB and yhjH</b><br> | |
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| - | <b>Biobrick Standardization of motA, motB and yhjH</b><br> | + | |
<ul> | <ul> | ||
<li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | <li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | ||
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| - | <h2><b> | + | <h2><b>V09_05 </b></h2><br> |
| - | <b>Double Digest of motA, motB, yhjH and Psb1c3</b><br> | + | <b>V09_05_1 Double Digest of motA, motB, yhjH and Psb1c3</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li> | <li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li> | ||
</ul> | </ul> | ||
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<br> | <br> | ||
| - | + | <b>V09_05_2 Quickchange of FliC</b><br> | |
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| - | <b>Quickchange of FliC</b><br> | + | |
<ul> | <ul> | ||
<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li> | <li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li> | ||
</ul> | </ul> | ||
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| - | <h2><b> | + | <h2><b>V09_06 </b></h2><br> |
<b>Ligation and Transformation of motA, motB and yhjH</b><br> | <b>Ligation and Transformation of motA, motB and yhjH</b><br> | ||
<ul> | <ul> | ||
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