Team:Goettingen/week9-2

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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div>
 
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<li class="toclevel-1"><a href="#week9"><span class="tocnumber">1</span> <span class="toctext">9th week (25th June to 01st July)</span></a></li>
 
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<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week9#1"><span class="tocnumber"></span> <span class="toctext">#1 Selection / Swimming</span></a></li>
 
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<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week9#2"><span class="tocnumber"></span> <span class="toctext">#2 Speed Improvement</span></a></li>
 
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<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week9#3"><span class="tocnumber"></span> <span class="toctext">#3 Chemoreceptor Library</span></a></li>
 
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<h2><b><a name="week9#1">#1 Selection / Swimming</a></b></h2>
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<h2><b><a name="week8#2">#2 Speed Improvement - 8th week</a></b></h2>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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Under process..
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<h2><b>V06_18 </b></h2><br>
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<b>Chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i>(DH10B)</i></b><br>
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<li>Experiment: <br> </li>
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<li>Experiment: <br>  
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In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i>(DH10B) as described in the protocol. <br>
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<li>Observations & Results: <br> </li>
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The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li>
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<h2><b><a name="week9#2">#2 Speed Improvement</a></b></h2>
 
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
 
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Under process...
 
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<h2><b>V06_19 </b></h2><br>
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<b>V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B) </b><br>
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Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li>
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The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.
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<b>V06_19_2 Repetition of the chemical transformartion of the <i>araC</i>-promoter into <i>E. coli</i> (DH10B) </b><br>
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Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol. <br>  
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The transformation was again not successful. Only the positive control showed any growth.  </li>
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<h2><b><a name="week9#3">#3 Chemoreceptor Library</a></b></h2>
 
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
 
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<h2><b>V06_20 </b></h2><br>
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<b> Preparation of over night cultures</i></i></b><br>
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Over night cultures were prepared for group 1 and group 3:<br>
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20E-<i>tar</i> <br>
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18M-<i>tar</i> <br>
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19C-<i>tar</i> <br>
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Revision as of 10:30, 15 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week9-2"