Team:UCSF/Bootcamp

9-10 am Introductions and Overview of UCSF iGEM Program (VERONICA)
10-11 am Introduction to Synthetic Biology/What is Synthetic Biology
11-12 Keeping an online lab notebook with Syapse (SEP)
1-3pm Basic Lab Review (VERONICA and SAMI)
- Using BLAST and Ape to find and look at gene sequences/plasmid
- Reading a Scientific Paper
- Staying Organized (labeling, keeping your notebook)
3-4pm Team assignments

Tuesday June 12: (GH-S202)

10-10:30am Introduction to Model Organisms (VERONICA)
11-12pm E. Coli - Growth, Maintenance, and Transformation procedures (IAN)
Common promoters and types of promoters (inducible, strong, leaky, weak, etc).
1-3pm Lecture and Discussion: Microbial Interactions (IAN)
3-4pm Yeast Mating and Genetics (VERONICA)

Wednesday June 13: (GH-S202)

9:45 am Meet in S202, walk over to Gladstone(VERONICA)
10-11am Lab Safety Training in Gladstone, Conf Room 264 A&B (HENK MAR)
12:30-1pm Review of standard restriction digest-ligation cloning and PCR (VERONICA)
1-2:30pm Lecture on Gibson Assembly (JARED)
2:30-4pm Primer Design and Cloning Workshop (VERONICA + Buddies/Jared)

Thursday June 14 (GH-S202)

9:30-11am Paper Review and Discussion – Microbial Interactions (VERONICA)
11-12:30 Computational Biology (GRAHAM + BEN)
2:00-4 pm Lecture on Flow Cytometry (PHIL)

Friday June 15 (GH-S204)

9-10 Using Pubmed and Publications Tutorial (CONNIE)
10:00-11:30 Paper Review and Discussion – Synthetic Mutualism and Tunable Symbiosis (VERONICA)
1-3 pm Visit to Core Facilities , Gladstone Institutes
3-4:30pm Computational Modeling of Biological Systems (GRAHAM + BEN)

Monday June 18, BH-212

10-12 Review of week 1, introduction of other relevant topics(VERONICA)
1-4 pm Brainstorming exercise and team building activity

Tuesday June 19, BH-212

10-12 Project Brainstorming Day #1
1-4 Break into Teams, develop project ideas

Wednesday June 20, GH-S261

10-12 Project Brainstorming Day #2
1-4pm Break into teams, further develop project ideas, put together presentation/proposal

Thursday June 21, GH-S261

9-11am Finalize presentations
11am Presentation of Project Proposals
12pm Lunch (provided)
1-4pm Decision on final project(s), break down project(s) into smaller groups

Friday June 22, GH-S261

Begin organizing and preparing for lab work (order primers, start growing strains, etc)