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Team:Goettingen/week7-2
From 2012.igem.org
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | ||
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<table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | ||
<tr bordercolor="black" valign="top"> | <tr bordercolor="black" valign="top"> | ||
<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
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<h2><b>V06_11 </b></h2><br> | <h2><b>V06_11 </b></h2><br> | ||
<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> | <b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> | ||
| - | <ul> | + | |
| - | <li>Experiment: <br> | + | <ul> |
| + | <li>Experiment: <br> | ||
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | ||
| - | </ul> | + | </ul> |
<br> | <br> | ||
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<b>V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)</b><br> | <b>V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)</b><br> | ||
| - | <ul> | + | <ul> |
| - | <li>Experiment: <br> | + | <li>Experiment: <br> |
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:<br> | For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:<br> | ||
20G - #1 <br> | 20G - #1 <br> | ||
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18O - #2 <br> | 18O - #2 <br> | ||
18O - #3 <br> | 18O - #3 <br> | ||
| - | 18K - #1 <br> | + | 18K - #1 <br> |
18K - #2 <br> | 18K - #2 <br> | ||
18K - #3 <br></li> | 18K - #3 <br></li> | ||
| - | <li>Observations & Results: <br> | + | <li>Observations & Results: <br> |
The retransformation was successful since all plates showed numeours colonoes except the negative control. | The retransformation was successful since all plates showed numeours colonoes except the negative control. | ||
| - | </ul> | + | </ul> |
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<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
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<b>Preparation of over night cultures</i></b><br> | <b>Preparation of over night cultures</i></b><br> | ||
<ul> | <ul> | ||
| - | <li>Experiment: <br> | + | <li>Experiment: <br> |
In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br> | In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br> | ||
20E - #2 <br> | 20E - #2 <br> | ||
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<tr bordercolor="black" valign="top"> | <tr bordercolor="black" valign="top"> | ||
<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
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<h2><b>V06_13 </b></h2><br> | <h2><b>V06_13 </b></h2><br> | ||
<b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> | <b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> | ||
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | + | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li> |
<br> | <br> | ||
<br> | <br> | ||
<b>V06_13_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br> | <b>V06_13_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br> | ||
| - | </ul><ul> | + | </ul> |
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| + | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | For the chemical transformation the standard protocol was followed. <br> | + | For the chemical transformation the standard protocol was followed. <br> |
<li>Observations & Results: <br> | <li>Observations & Results: <br> | ||
The transformation was successful since all plates showed numeours colonoes except the negative control.</li> | The transformation was successful since all plates showed numeours colonoes except the negative control.</li> | ||
| - | </ul><br></td></tr> | + | </ul><br> |
| + | |||
| + | </td></tr> | ||
</table> | </table> | ||
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<b> Preparation of over night cultures</i></i></b><br> | <b> Preparation of over night cultures</i></i></b><br> | ||
<ul> | <ul> | ||
| - | <li>Experiment: <br> | + | <li>Experiment: <br> |
Over night cultures of the pBAD-<i>sfGFP</i> clones were prepared in order to isolate the plasmids. <br> | Over night cultures of the pBAD-<i>sfGFP</i> clones were prepared in order to isolate the plasmids. <br> | ||
<br></td></tr> | <br></td></tr> | ||
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<b> Miniprep of pBAD-<i>sfGFP</i></i></i></b><br> | <b> Miniprep of pBAD-<i>sfGFP</i></i></i></b><br> | ||
<ul> | <ul> | ||
| - | <li>Experiment: <br> | + | <li>Experiment: <br> |
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br> | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br> | ||
<br></td></tr> | <br></td></tr> | ||
Revision as of 11:12, 14 September 2012
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