Team:Goettingen/week7-2

V06_11

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

• Experiment:
  For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
  20G - #1
  20G - #2
  2G - #1
  2G - #2
  2G - #3
  20I - #2
  20I - #3
  18O - #2
  18O - #3
  18K - #1
  18K - #2
  18K - #3
  
• Observations & Results:
  The retransformation was successful since all plates showed numeours colonoes except the negative control.

V06_12

• Experiment:
  In order to gain further plasmid material over night cultures of one colonie of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.
  20E - #2
  20G - #1
  2G - #1
  20I - #2
  18M - #2
  18O - #2
  18K - #1
  18C - #2
  
  

V06_13

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
• 
  V06_13_2 Chemical transformartion of pBAD-sfGFP into E. coli (DH10B)
  
  • Experiment:
    For the chemical transformation the standard protocol was followed.
    
  • Observations & Results:
    The transformation was successful since all plates showed numeours colonoes except the negative control.
  
  

V06_14

• Experiment:
  Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.
  
  

V06_15

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.