Team:Goettingen/week6-2

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Chemical retransformartion of the flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
20E - #2
20E - #3
18M - #2
18M - #3
18C - #1
18C - #2
Observations & Results:
The retransformation was successful since all plates showed numeours colonoes except the negative control.

V06_05_1 Preparative double digestion of flhDC and plasmids containing further promoter

V06_05_2 Insertion of flhDC into the BioBrick plamids

Experiment:
The ligation of the digested flhDC with the BioBrick plasmids was conducted as described in the protocol.

V06_06_1 Chemical transformartion of the flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical transformation the standard protocol was followed.
Observations & Results:
The transformation was successful since all plates showed numeours colonoes except the negative control.However, on the plastes with 18K and 18O few red colonies could be observed. These were again rejected.

V06_06_2 Preparation of over night cultures

Experiment:
Over night cultures were preaped of three colonies of each constructs in order to isolate the plasmids.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <br>
 <table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b>V05_27 </b></h2><br>
+<h2><b>V06_06 </b></h2><br>
-<b>Preparation of over night cultures</i></b><br>
+<b>V06_06_1 Chemical transformartion of the <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br>
 <ul>
-<li>Experiment: <br>
+<li>Experiment:  <br>
-In order to isolate the plasmids, over night cultures of the <i>E. coli</i> (DH10B) cells transformed with the different constitutive promoters were prepared.</li>
+For the chemical transformation the standard protocol was followed.
-<br> </li>
+<li>Observations & Results: <br>
+The transformation was successful since all plates showed numeours colonoes except the negative control.However, on the plastes with 18K and 18O few red colonies could be observed. These were again rejected.
+</ul>
+<br>
+<b>V06_06_2  Preparation of over night cultures</b><br>
+<ul>
+<li>Experiment:  <br>
+Over night cultures were preaped of three colonies of each constructs in order to isolate the plasmids.<br> </li>
 </ul>
 <br></td></tr>
 </table>
 <br>