Team:Goettingen/week7-3

From 2012.igem.org

(Difference between revisions)
Line 539: Line 539:
<ul>
<ul>
<li>Experiment: <br>We selected 8 different promoters for our chemoreceptor TAR. We chose the following parts for this purpose: <br>  
<li>Experiment: <br>We selected 8 different promoters for our chemoreceptor TAR. We chose the following parts for this purpose: <br>  
-
J23100 <br> J23104 <br> J23105 <br> J23106 <br> J23109 <br> J23112 <br> J23113 <br> J23114 <br> all of these in vector J61002 (for details click <a href="http://partsregistry.org/Main_Page">here</a>).
+
J23100 (18C) <br> J23104 (18K) <br> J23105 (18M) <br> J23106 (18O) <br> J23109 (2G) <br> J23112 (20E) <br> J23113 (20G) <br> J23114 (20I) <br> all of these in vector J61002 (for details click <a href="http://partsregistry.org/Main_Page">here</a>). These constructs were, prior to this experiment, transformed into <i>E. coli</i>, prepped and stored at -20 °C. <br>
 +
The vector J61002 was digested with <i>Spe</i>I and <i>Pst</i>I, the insert TAR_QC was digested with <i>Xba</i>I <i>Pst</i>I according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.
</li>
</li>
</ul>
</ul>

Revision as of 08:46, 14 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week7-3"