Team:Goettingen/week5-3

From 2012.igem.org

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<li>Experiment: <br>DNA was prepped using PeqGOLD MiniPrep Kit (Peqlab). A DNA test digest with <i>Eco</i>RI and <i>Xba</i>I was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> and analyzed on a 1% agarose gel. Test digest showed 8 out of 10 clones contained the construct pUC18_TAR. QuikChange PCR was performed using designed primers and <i>Pfu</i> Turbo polymerase (see QuikChange protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#QuikChange_Protocol">here</a>).
<li>Experiment: <br>DNA was prepped using PeqGOLD MiniPrep Kit (Peqlab). A DNA test digest with <i>Eco</i>RI and <i>Xba</i>I was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> and analyzed on a 1% agarose gel. Test digest showed 8 out of 10 clones contained the construct pUC18_TAR. QuikChange PCR was performed using designed primers and <i>Pfu</i> Turbo polymerase (see QuikChange protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#QuikChange_Protocol">here</a>).
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<h2><b>V06_02 </b></h2><br>
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<b>Changing the <i>Xba</i>I site in the <i>tar</i> sequence via QuikChange</b><br>
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<li>Experiment: <br><i>Dpn</i>I digest of QuikChange PCR product to get rid of the parent plasmid.
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Revision as of 13:41, 12 September 2012

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