Team:Goettingen/week5-3

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Revision as of 13:25, 12 September 2012

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Insertion of tar into pUC18

Experiment:
Double digest of tar and pUC18 using EcoRI, PstI and Orange buffer (ThermoScientific) according to protocol. Gel extraction and purification of pUC18 and tar using PeqGOLD Gelextraction Kit (Peqlab). Ligation of tar into pUC18 using T4 DNA Ligase (Thermo Scientific).

Transformation of DH10B with pUC18_TAR construct

Experiment:
Transformation was carried out according to protocol. Transformed DH10B were plated on LB-Amp plates (see here for recipe). Transformation was successful.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <b>Transformation of DH10B with pUC18_TAR construct</b><br>
 <ul>
-<li>Experiment: <br>Transformation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Transformed DH10B were plated on LB-Amp plates (see here for <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>. Transformation was successful.
+<li>Experiment: <br>Transformation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Transformed DH10B were plated on LB-Amp plates (see here for <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>). Transformation was successful.
 </li>
 </ul>