Team:Goettingen/week5-3

From 2012.igem.org

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<li>Experiment: <br>Double digest of <i>tar</i> and pUC18 using <i>Eco</i>RI, <i>Pst</i>I and Orange buffer (ThermoScientific) according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Gel extraction and purification of pUC18 and <i>tar</i> using PeqGOLD Gelextraction Kit (Peqlab). Ligation of <i>tar</i> into pUC18 using T4 DNA Ligase (Thermo Scientific).  
<li>Experiment: <br>Double digest of <i>tar</i> and pUC18 using <i>Eco</i>RI, <i>Pst</i>I and Orange buffer (ThermoScientific) according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Gel extraction and purification of pUC18 and <i>tar</i> using PeqGOLD Gelextraction Kit (Peqlab). Ligation of <i>tar</i> into pUC18 using T4 DNA Ligase (Thermo Scientific).  
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<h2><b>V05_30 </b></h2><br>
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<b>Transformation of DH10B with pUC18_TAR construct</b><br>
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<li>Experiment: <br>Transformation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Transformed DH10B were plated on LB-Amp plates (see here for <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>. Transformation was successful.
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Revision as of 13:24, 12 September 2012

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