Team:Goettingen/week5-3

From 2012.igem.org

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<h2><b>VX_Y </b></h2><br>
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<h2><b>V05_29 </b></h2><br>
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<b>Titel</b><br>
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<b>Insertion of <i>tar</i> into pUC18</b><br>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: <br>Double digest of <i>tar</i> and pUC18 using <i>Eco</i>RI, <i>Pst</i>I and Orange buffer (ThermoScientific) according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Gel extraction and purification of pUC18 and <i>tar</i> using PeqGOLD Gelextraction Kit (Peqlab). Ligation of <i>tar</i> into pUC18 using T4 DNA Ligase (Thermo Scientific).
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Revision as of 13:18, 12 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week5-3"