Team:UC Chile2/General Protocols

From 2012.igem.org

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<p>For a 375ul 1.33X Gibson Assembly Master Mix</p>
<p>For a 375ul 1.33X Gibson Assembly Master Mix</p>
<li>100uL 5X Gibson Assembly Isothermal Buffer</li>
<li>100uL 5X Gibson Assembly Isothermal Buffer</li>
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<li>6.25uL Phusion Polymerase XXX U/uL (cat N° F-350S) from Thermo Scientific </li>
+
<li>6.25uL Phusion Polymerase 2 U/uL (cat N° F-350S) from Thermo Scientific </li>
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<li>2uL Epicentre T5 Exonuclease 1U/ul(cat N° T5E4111K) from Illumina</li>
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<li>2uL Epicentre T5 Exonuclease 1U/uL (cat N° T5E4111K) from Illumina</li>
<p>It is important to dilute the T5 Exonuclease stock from 10U/uL to 1U/ul to measure the volume correctly</p>
<p>It is important to dilute the T5 Exonuclease stock from 10U/uL to 1U/ul to measure the volume correctly</p>
<li>50uL Taq DNA Ligase 2000 U/uL (cat N° M208S) from NEB</li>
<li>50uL Taq DNA Ligase 2000 U/uL (cat N° M208S) from NEB</li>

Revision as of 03:20, 12 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012