Team:TU Darmstadt/Materials/Destain
From 2012.igem.org
(Difference between revisions)
(→Destain) |
(→Destain) |
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Line 46: | Line 46: | ||
* 500mL Methanol | * 500mL Methanol | ||
* 100mL Acetic Acid | * 100mL Acetic Acid | ||
- | * 400mL | + | * 400mL dH<sub>2</sub>O |
=== Application === | === Application === | ||
* SDS page gel is placed in a small tub and covered in destain solution | * SDS page gel is placed in a small tub and covered in destain solution |
Revision as of 21:48, 11 September 2012
Contents |
Destain
About
The following destain solution is used for coomassie dyed SDS pages.
Ingredients
Mix together the following contents under a fume hood:
- 500mL Methanol
- 100mL Acetic Acid
- 400mL dH2O
Application
- SDS page gel is placed in a small tub and covered in destain solution