Team:TU Darmstadt/Materials/Destain

From 2012.igem.org

(Difference between revisions)
(Destain)
(Destain)
Line 46: Line 46:
* 500mL Methanol
* 500mL Methanol
* 100mL Acetic Acid
* 100mL Acetic Acid
-
* 400mL dH2O
+
* 400mL dH<sub>2</sub>O
=== Application ===
=== Application ===
* SDS page gel is placed in a small tub and covered in destain solution
* SDS page gel is placed in a small tub and covered in destain solution

Revision as of 21:48, 11 September 2012

Contents

Destain

About

The following destain solution is used for coomassie dyed SDS pages.

Ingredients

Mix together the following contents under a fume hood:

  • 500mL Methanol
  • 100mL Acetic Acid
  • 400mL dH2O

Application

  • SDS page gel is placed in a small tub and covered in destain solution