Team:Goettingen/week3-3

From 2012.igem.org

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<li>Experiment: <br>Preparation of LB medium and LB-Amp plates (recipe see <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">here</a>). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.
<li>Experiment: <br>Preparation of LB medium and LB-Amp plates (recipe see <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">here</a>). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.
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<h2><b>V05_16 </b></h2><br>
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<b>Isolation of pUC18 plasmid DNA</b><br>
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<li>Experiment: <br>Isolation of pUC18 from DH10B using PeqGOLD MiniPrep Kit (Peqlab).
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<h2><b>V05_18 </b></h2><br>
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<b>Insertion of <i>tar</i> into pUC18</b><br>
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<li>Experiment: <br>Double digest of <i>tar</i> and pUC18 using <i>Eco</i>RI, <i>Pst</i>I and Orange Buffer (ThermoScientific). Preparation of chemical competent DH10B cells for transformation.
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Revision as of 20:01, 11 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week3-3"