Team:Goettingen/week1-2

From 2012.igem.org

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<h2><b><a name="week3#2">#3 tetsttetstetstetsteste - 1st week</a></b></h2>
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<h2><b><a name="week3#2">#2 Speed Improvement - 1st week</a></b></h2>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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<h2><b>V5_04 </b></h2><br>
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<h2><b>V05_04_1 </b></h2><br>
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<b>TITEL</b><br>
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<b>Mini Prep of Psb1c3 (including motA or MotB)</b><br>
<ul>
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<li>Experiment: <br>TEXTXTTXTuick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li>
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<li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li>
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<h2><b>V5_04 </b></h2><br>
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<h2><b>V09_10_2 </b></h2><br>
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<b>TITEL</b><br>
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<b>Test digestion of Psb1c3 (including motA and motB)</b><br>
<ul>
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<li>Experiment: <br>TEXTXTTXTuick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li>
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<li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li>
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<h2><b>V09_4_1 </b></h2><br>
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<h2><b>V09_10_3 </b></h2><br>
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<b>Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br>
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<b>Digestion of motA, motB and the promoters 20E, 20I and 18C</b><br>
<ul>
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<li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li>
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<li>Experiment: <br> MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).</li>
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<h2><b>V09_4_2 </b></h2><br>
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<h2><b>V09_10_4 </b></h2><br>
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<b>Biobrick Standardization of motA, motB and yhjH</b><br>
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<li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li>
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<h2><b>V09_5_1 </b></h2><br>
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<b>Double Digest of motA, motB, yhjH and Psb1c3</b><br>
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<li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li>
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<h2><b>V09_5_2 </b></h2><br>
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<b>Quickchange of FliC</b><br>
<b>Quickchange of FliC</b><br>
<ul>
<ul>
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<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li>
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<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed</li>
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<h2><b>V09_6 </b></h2><br>
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<h2><b>V09_11_1 </b></h2><br>
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<b>Ligation and Transformation of motA, motB and yhjH</b><br>
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<b>Ligation and Transformation of motA, motB</b><br>
<ul>
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<li>Experiment: <br> After the ligation of motA, motB and yhjH into the plasmid Psb1c3, the ligation products were transformed into the E. coli strain DH10B.</li>
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<li>Experiment: <br> After the ligation of motA and motB into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.</li>
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Revision as of 14:08, 11 September 2012

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