Team:Goettingen/week19-2

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Revision as of 12:29, 11 September 2012

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#2 Speed Improvement - 19th week

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V09_3

Quickchange of FliC

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.

V09_4_1

Test digestion of Psb1c3(including FlHDC under the control of different promoters) with

Experiment:
Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

V09_4_2

Biobrick Standardization of motA, motB and yhjH

Experiment:
PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).

V09_5_

Quickchange of FliC

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed

V09_11_1

Ligation and Transformation of motA, motB

Experiment:
After the ligation of motA and motB into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.

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