Team:Goettingen/week20-2

(Difference between revisions)

Revision as of 11:57, 11 September 2012

Language:

English,

Deutsch

Mini Prep pf Psb1c3 (including motA or MotB)

Test digestion of Psb1c3 (including motA and motB)

Experiment:
Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

Digestion of motA, motB and the promoters 20E, 20I and 18C

Experiment:
MotA and MotB were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 536: / Line 536: @@
 <td width="900" bordercolor="black" valign="top">
 <h2><b>V09_10_1 </b></h2><br>
-<b>Test digestion of Psb1c3 (including motA and motB)</b><br>
+<b>Mini Prep pf Psb1c3 (including motA or MotB)</b><br>
 <ul>
-<li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones</li>
+<li>Experiment: <br>Plasmids werde prepped using PeqGOLD MiniPrep Kit (Peqlab)</li>
 </ul>
 <br></td></tr>
@@ Line 547: / Line 547: @@
 <td width="900" bordercolor="black" valign="top">
 <h2><b>V09_10_2 </b></h2><br>
+<b>Test digestion of Psb1c3 (including motA and motB)</b><br>
+<ul>
+<li>Experiment: <br>Test digestion of Psb1c3 (including motA or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li>
+</ul>
+<br></td></tr>
+</table>
+<br>
+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
+<h2><b>V09_10_3 </b></h2><br>
 <b>Digestion of motA, motB and the promoters 20E, 20I and 18C</b><br>
 <ul>