"
Team:Freiburg/Project/Experiments
From 2012.igem.org
(Difference between revisions)
| Line 50: | Line 50: | ||
To show the functinality of our TAL protein as well as the function of the VP 64 transcriptionfactor fusion protein we used a TAL-VP64 fusion construct to target a minimal promotor coupled with EGFP | To show the functinality of our TAL protein as well as the function of the VP 64 transcriptionfactor fusion protein we used a TAL-VP64 fusion construct to target a minimal promotor coupled with EGFP | ||
| + | |||
| + | {|align="center" | ||
| + | |[[Image:Plasmids.png|500px|no frame]] | ||
| + | |} | ||
| + | |||
| + | |||
| + | The Experiment was done with four different transfections, either no plasmid, only the TAL vektor, only the testplasmid or a cotransfection of both plasmids. | ||
| + | |||
| + | |||
| + | {|align="center" | ||
| + | |[[Image:Exp1.png|350px|no frame]] | ||
| + | |} | ||
Revision as of 22:53, 9 September 2012
|
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
|
| Building a Toolkit | Using the Toolkit | Experiments |
|---|
Gene activation
To show the functinality of our TAL protein as well as the function of the VP 64 transcriptionfactor fusion protein we used a TAL-VP64 fusion construct to target a minimal promotor coupled with EGFP
|
The Experiment was done with four different transfections, either no plasmid, only the TAL vektor, only the testplasmid or a cotransfection of both plasmids.
|
