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Template:Team:Edinburgh/Notebook/Week06
From 2012.igem.org
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<b> PCR for Shewanella </b><br/> | <b> PCR for Shewanella </b><br/> | ||
Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB. <br/> | Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB. <br/> | ||
| - | <img style="width:350px;" src=" | + | <img style="width:350px;" src="http://i1056.photobucket.com/albums/t366/edigem12/SkitchImage0-2.png" /><img style="width:350px;" src="http://i1056.photobucket.com/albums/t366/edigem12/SkitchImage0-3.png" /> |
</p> | </p> | ||
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<b> Kod PCR for Shewanella </b><br/> | <b> Kod PCR for Shewanella </b><br/> | ||
Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day. </b> | Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day. </b> | ||
| - | <img style="width:612px;" src=" | + | <img style="width:612px;" src="http://i1056.photobucket.com/albums/t366/edigem12/SkitchImage0-4.png" /></br><br/> |
<b> Half-cell experiment </b><br/> | <b> Half-cell experiment </b><br/> | ||
2 new half-cells were assembled and set for autoclaving | 2 new half-cells were assembled and set for autoclaving | ||
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<p> | <p> | ||
<b> PCR for Shewanella </b><br/> | <b> PCR for Shewanella </b><br/> | ||
| - | Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA | + | Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA, mtrA, mtrCAB samples were purified.<br/> |
| + | <img style="width:612px;" src="http://i1056.photobucket.com/albums/t366/edigem12/SkitchImage0-5.png" /></br><br/> | ||
</p> | </p> | ||
</div><!-- /article-text --> | </div><!-- /article-text --> | ||
Revision as of 15:44, 1 August 2012
Half-cell results
Half cell voltage increased 10-fold to 50 mV
PCR for Shewanella
Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB.
Kod PCR for Shewanella
Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day.
Half-cell experiment
2 new half-cells were assembled and set for autoclaving
PCR for Shewanella
Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA, mtrA, mtrCAB samples were purified.
