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Template:Team:Edinburgh/Notebook/Week06
From 2012.igem.org
(Difference between revisions)
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| - | < | + | <b> Half-cell results </b><br/> |
| + | Half cell voltage increased 10-fold to 50 mV<br/><br/> | ||
| + | <b> PCR for Shewanella </b><br/> | ||
| + | Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB. <br/> | ||
| + | |||
</p> | </p> | ||
</div><!-- /article-text --> | </div><!-- /article-text --> | ||
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<p> | <p> | ||
| - | + | <b> Kod PCR for Shewanella </b><br/> | |
| + | Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day. </b> | ||
| + | <img style="width:612px;" src="https://www.evernote.com/shard/s109/res/574c3de7-8ae3-470a-b2ee-fa2672c401e3/SkitchImage0.png" /></br><br/> | ||
| + | <b> Half-cell experiment </b><br/> | ||
| + | 2 new half-cells were assembled and set for autoclaving | ||
</p> | </p> | ||
</div><!-- /article-text --> | </div><!-- /article-text --> | ||
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<div class="entry-text"> | <div class="entry-text"> | ||
<p> | <p> | ||
| - | < | + | <b> PCR for Shewanella </b><br/> |
| + | Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA sample from previous raction was purified. | ||
</p> | </p> | ||
</div><!-- /article-text --> | </div><!-- /article-text --> | ||
Revision as of 10:53, 1 August 2012
Half-cell results
Half cell voltage increased 10-fold to 50 mV
PCR for Shewanella
Further PCR was performed for confirmed Shewanella oneidensis using Taq protocol and 3 sets of primers: cymA, mtrA and mtrCAB.
Kod PCR for Shewanella
Kod PCR was performed for confirmed Shewanella oneidensis using 3 sets of primers: cymA, mtrA and mtrCAB. Due to PCR machine error the reaction was repeated for MtrA and MtrCAB on the following day.
Half-cell experiment
2 new half-cells were assembled and set for autoclaving
PCR for Shewanella
Kod PCR was repeated for MtrA and MtrCAB in confirmed S. oneidensis. CymA sample from previous raction was purified.
