Team:NTNU Trondheim/Constructs

From 2012.igem.org

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Test and working constructs:
Test and working constructs:
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Intermediates (constructs that have no function alone):
 
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|K+RBS+GFP+DTT||<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_K082003</partinfo>,<partinfo>BBa_B0015</partinfo>||GFP test construct||?||?||Negative results  
|K+RBS+GFP+DTT||<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_K082003</partinfo>,<partinfo>BBa_B0015</partinfo>||GFP test construct||?||?||Negative results  
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|PBAD+lysis|| <partinfo>BBa_K206000</partinfo>, <partinfo>BBa_K112808</partinfo>||Arabinoseindusert promotor med lysis device|| <partinfo>pSB1A3</partinfo> || ||Testet og fungerer
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Intermediates (constructs that have no function alone):
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! Navn ||  BioBricks (5'-3') ||  Funksjon || Backbone (length)  || Copy number ||Status
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|DLD+RBS|| <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Laktatpromotor med RBS||<partinfo>pSB1A2</partinfo> (2079)|| High ||Testkuttet
|DLD+RBS|| <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Laktatpromotor med RBS||<partinfo>pSB1A2</partinfo> (2079)|| High ||Testkuttet
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|GFP(+LVA)+DTT ||<partinfo>BBa_K082003</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo>  || ||Testkuttet
|GFP(+LVA)+DTT ||<partinfo>BBa_K082003</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo>  || ||Testkuttet
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|PBAD+lysis|| <partinfo>BBa_K206000</partinfo>, <partinfo>BBa_K112808</partinfo>||Arabinoseindusert promotor med lysis device|| <partinfo>pSB1A3</partinfo> || ||Testet og fungerer
 
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|LuxR+DTT|| <partinfo>BBa_C0062</partinfo>, <partinfo>BBa_B0015</partinfo>||LuxR-gen med terminator||<partinfo>pSB1AK3</partinfo> || ||Ligert og transformert
|LuxR+DTT|| <partinfo>BBa_C0062</partinfo>, <partinfo>BBa_B0015</partinfo>||LuxR-gen med terminator||<partinfo>pSB1AK3</partinfo> || ||Ligert og transformert

Revision as of 22:11, 28 July 2012

Search and Destroy
Search and Destroy
NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze
</div>

Test and working constructs:


Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
pllD pllD promoter in pSB1C3<partinfo>pSB1C3</partinfo>HighTransformed but unverified
VGB+RBS+YFP+DTT<partinfo>BBa_K561001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>VGB test construct??Transformed. Untested.
VHB+RBS+YFP+DTT<partinfo>BBa_K258005</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>VHB test construct??Transformed. Untested.
K+RBS+RFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E1010</partinfo>,<partinfo>BBa_B0015</partinfo>RFP test construct??Negative results
K+RBS+YFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>YFP test construct??Works
K+RBS+GFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_K082003</partinfo>,<partinfo>BBa_B0015</partinfo>GFP test construct??Negative results
PBAD+lysis <partinfo>BBa_K206000</partinfo>, <partinfo>BBa_K112808</partinfo>Arabinoseindusert promotor med lysis device <partinfo>pSB1A3</partinfo> Testet og fungerer


Intermediates (constructs that have no function alone):

Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
DLD+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Laktatpromotor med RBS<partinfo>pSB1A2</partinfo> (2079) High Testkuttet
JEN1+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Laktatpromotor med RBS<partinfo>pSB1A2</partinfo> (2079) High Testkuttet
VGB+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Oksygenpromotor med RBS <partinfo>pSB1A2</partinfo> (2079) High Testkuttet
VHB+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Oksygenpromotor med RBS <partinfo>pSB1A2</partinfo> (2079) High Testkuttet
RFP+DTT <partinfo>Bba_E1010</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
YFP+DTT <partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
CFP+DTT <partinfo>BBa_E0020</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
GFP(+LVA)+DTT <partinfo>BBa_K082003</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> Testkuttet
LuxR+DTT <partinfo>BBa_C0062</partinfo>, <partinfo>BBa_B0015</partinfo>LuxR-gen med terminator<partinfo>pSB1AK3</partinfo> Ligert og transformert
LuxI+DTT <partinfo>BBa_C0061</partinfo>, <partinfo>BBa_B0015</partinfo>LuxI-gen med terminator<partinfo>pSB1AK3</partinfo> Ligert og transformert
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