Team:NTNU Trondheim/Stocks
From 2012.igem.org
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(→Restriction digests and ligation mixtures) |
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! Name/abbreviation ||Date stored|| Description ||Enzymes|| | ! Name/abbreviation ||Date stored|| Description ||Enzymes|| | ||
|- | |- | ||
- | |YFP+Term (religation)||11.07||?|YFP+term (religated)||? | + | |YFP+Term (religation)||11.07||?|YFP+term (religated)||?||<partinfo>pSB1AK3</partinfo> |
|- | |- | ||
|GFP+Term (religation)||11.07||<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo>|| ||<partinfo>pSB1AK3</partinfo> | |GFP+Term (religation)||11.07||<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo>|| ||<partinfo>pSB1AK3</partinfo> |
Revision as of 20:07, 28 July 2012
This page lists stored cultures and other samples:
See also Constructs page.
Contents |
Stored samples
Resuspended DNA
(Dried DNA from distribution kit resuspended in dH2O)
Name/abbreviation | Date stored | Description | Source | Backbone |
---|---|---|---|---|
LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
Lysis | 22.06 | BBa_K112808 | (RS) | <partinfo>pSB1A2</partinfo> |
C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
C79 | ? | <partinfo>BBa_C0079</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
Isolated DNA
(Miniprep, gel extraction and PCR products) NOT included here: Digestion and ligation mixtures/products
Name/abbreviation | Date stored | Description | Concentration | Backbone |
---|---|---|---|---|
LuxR+Term | 16.7 | <partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,0 | <partinfo>pSB1AK3</partinfo> |
LuxI+Term | 16.7 | <partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,6 | <partinfo>pSB1AK3</partinfo> |
C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | <partinfo>pSB2K3</partinfo> |
C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | <partinfo>pSB2K3</partinfo> |
RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | <partinfo>pSB1A2</partinfo> |
LI (BBa_K092400) | ? | <partinfo>BBa_K092400</partinfo> | <partinfo>pSB1A2</partinfo> | |
DLD + RBS | 9.07 | <partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> | 24,1 | <partinfo>pSB1A2</partinfo> |
VHB + RBS | 9.07 | <partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> | 48,3 | <partinfo>pSB1A2</partinfo> |
Jen1 + RBS | 9.07 | <partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> | 35,3 | <partinfo>pSB1A2</partinfo> |
VGB + RBS | 9.07 | <partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> | 40,0 | <partinfo>pSB1A2</partinfo> |
LR | ? | <partinfo>BBa_C0062</partinfo> | 20,3 | <partinfo>pSB1A2</partinfo> |
VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | <partinfo>pSB1C3</partinfo> |
VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | <partinfo>pSB1C3</partinfo> | |
Constitutive promoter | 22.06 | <partinfo>BBa_J23119</partinfo> | 27,7 | <partinfo>pSB1A2</partinfo> |
DLD | 4.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | <partinfo>BBa_J63010</partinfo> |
K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> |
LuxR+HSL | 24.06 | <partinfo>BBa_R0062</partinfo> | <partinfo>pSB1A2</partinfo> | |
pBAD+lysis | 9.7 | 23,6 | <partinfo>pSB1A3</partinfo> | |
DNA lysis | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | |
D2 | 22.6 | <partinfo>BBa_K145150</partinfo> | 23,1 | <partinfo>pSB1A2</partinfo> |
RBS | 6.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | <partinfo>pSB1A2</partinfo> |
LuxI | 6.07 | <partinfo>BBa_C0061</partinfo> | 38,6 | <partinfo>pSB1A2</partinfo> |
VHB | 4.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | <partinfo>pSB1A2</partinfo> |
Jen1 | 4.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | <partinfo>BBa_J63010</partinfo> |
luxR+HSL | 25.06 | <partinfo>BBa_R0062</partinfo> | 13,3 | <partinfo>pSB1A2</partinfo> |
Lysis1 DNA | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | |
GFP+DTT | 13.07 | <partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo> | 41,1 | <partinfo>pSB1AK3</partinfo> |
YFP+DTT | 13.07 | <partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo> | 55,5 | <partinfo>pSB1AK3</partinfo> |
RFP+DTT | 13.07 | <partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | 40,9 | <partinfo>pSB1AK3</partinfo> |
CFP+DTT | 13.07 | <partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo> | 47,7 | <partinfo>pSB1AK3</partinfo> |
pBAD B1 | 1.07 | <partinfo>BBa_K206000</partinfo> | 24,0 | <partinfo>pSB1A3</partinfo> |
D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> |
pBAD B2 | 1.07 | <partinfo>Bba_K206000</partinfo> | 19 | <partinfo>pSB1A3</partinfo> |
Colicin | 4.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | <partinfo>pSB1A2</partinfo> |
VGB+RBS+YFP+DTT | 26.07 | |||
VHB+RBS+YFP+DTT | 26.07 | |||
EYFP+RBS+YFP+DTT | 26.07 | |||
pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | <partinfo>pSB1A2</partinfo> | |
RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | <partinfo>pSB1A2</partinfo> |
DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | <partinfo>pSB1AK3</partinfo> |
DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | <partinfo>pSB1AK3</partinfo> |
RFP K | 22.06 | <partinfo>BBa_E1010</partinfo> | <partinfo>pSB2K3</partinfo> | |
GFP AK | 22.06 | <partinfo>BBa_K082003</partinfo> | <partinfo>pSB1AK3</partinfo> | |
CFP A | 22.06 | <partinfo>BBa_E0020</partinfo> | <partinfo>pSB1A2</partinfo> | |
YFP AK | 22.06 | <partinfo>BBa_E0030</partinfo> | <partinfo>pSB1AK3</partinfo> |
Restriction digests and ligation mixtures
(Inserts and backbones, DNA treated with enzymes)
Name/abbreviation | Date stored | Description | Enzymes | |
---|---|---|---|---|
YFP+Term (religation) | 11.07 | YFP+term (religated) | ? | <partinfo>pSB1AK3</partinfo> |
GFP+Term (religation) | 11.07 | <partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
CFP+Term (religation) | 11.07 | <partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
RFP +Term (religation) | 11.07 | <partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> |
Glycerol stocks
(Cell cultures kept at -80 C)
Name/abbreviation | Date stored | Strain | Plasmid | Position |
---|---|---|---|---|
pTet mRFP (BBa_I13521) | 27.07 | DH5a | pSB1A2 | |
pTet GFP (BBa_I13522) | 27.07 | DH5a | pSB1A2 | |
EYFP + LVA (BBa_E0430) | 27.07 | DH5a | pSB1A2 | |
RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | pSB2K3 | |
VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | pSB1C3 | |
LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | pSB1A2 | |
pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | pSB1A3 | |
LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | pSB1AK3 | |
pBAD+Lysis | 16.07 | DH5a | pSB1A3 | |
DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | pSB1AK3 | |
D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | pSB1A2 | |
CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | pSB1A2 | |
YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | pSB1AK3 |