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- | {{:Team:Arizona State/Templates/main|title=|content= | + | {{:Team:Arizona_State/Template:Header}} |
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- | We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.
| + | <html> |
- | <center>[[Image:ASU_tips.png|400px]]</center>
| + | <h3> Arizona State University iGEM 2012 </h3> |
- | == Abstract ==
| + | </html> |
- | <p>Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.</p>
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- | ''[[Team:Arizona State/Project/Introduction|more]]''
| + | {| style="color:#903;background-color:#ffb310;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" |
| + | !align="center"|[[Team:Arizona_State|Home]] |
| + | !align="center"|[[Team:Arizona_State/Team|Team]] |
| + | !align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Arizona_State Official Team Profile] |
| + | !align="center"|[[Team:Arizona_State/Project|Project]] |
| + | !align="center"|[[Team:Arizona_State/Parts|Parts Submitted to the Registry]] |
| + | !align="center"|[[Team:Arizona_State/Modeling|Modeling]] |
| + | !align="center"|[[Team:Arizona_State/Notebook|Notebook]] |
| + | !align="center"|[[Team:Arizona_State/Safety|Safety]] |
| + | !align="center"|[[Team:Arizona_State/Attributions|Attributions]] |
| + | |} |
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- | == What is CRISPR? ==
| + | [[File:toaster.jpg|500px|center]] |
- | <p>'''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.</p>
| + | [[Image:AsuToaster1.jpg|100px|right|frame|This is an '''awesome''' toaster.]] |
- | <center>
| + | [[Image:Toaster 6.jpg|100px|left|frame|This is also a pretty cool toaster.]] |
- | [[Image:ASU Crispr basic.png|600px|Basic mechanism]]<br> | + | <br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /> |
- | A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. '''[[Team:Arizona State/Project/CRISPR|More information]]'''.
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- | </center> | + | |
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- | }}
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| + | These are toasters. They are not ours though :( |
These are toasters. They are not ours though :(