Team:KAIST Korea/Notebook Labnote/2012 10 temp

From 2012.igem.org

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<section id="10">
<section id="10">
<div class="date">October 10<sup>th</sup> 2012</div></br>
<div class="date">October 10<sup>th</sup> 2012</div></br>
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<div id="content_note" >
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</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
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<span id="little">No Special Event!</span>
+
 +
<div class="note-title">LuxI and Xis OE template preparation</div>
 +
 
 +
<div id="content_note" >
 +
<b>Results</b></br></br>
 +
 
 +
<div align="center"><img id="figure" alt="2_1010Fig1" src="https://static.igem.org/mediawiki/2012/3/39/KAIST_LABNOTE101012_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 
</br>
</br>
 +
</div>
 +
</br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
 +
<div class="note-title">pAutoIntegrase cloning check and Xis template vector prep</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1010Fig2" src="https://static.igem.org/mediawiki/2012/4/4f/KAIST_LABNOTE101012_Fig2.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
<span id="little">pAutoIntegrase 1-10 – HindIII cut</br></span>
 +
<span id="little">pGEM-Xis 1-2 – EcoRI cut</br></span>
 +
 +
 +
</br>
</div>
</div>
 +
 +
</br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
 +
<div class="note-title">insert DNA preparation with OE PCR</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1010Fig3" src="https://static.igem.org/mediawiki/2012/d/df/KAIST_LABNOTE101012_Fig3.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
<span id="little">┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)</span></br>
 +
 +
</br>
 +
<div align="center"><img id="figure" alt="2_1010Fig4" src="https://static.igem.org/mediawiki/2012/7/7d/KAIST_LABNOTE101012_Fig4.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
<span id="little">┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)</span>
 +
 +
</br>
 +
</div>
 +
 +
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="11">
<section id="11">
<div class="date">October 11<sup>th</sup> 2012</div></br>
<div class="date">October 11<sup>th</sup> 2012</div></br>
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<div id="content_note" >
+
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
-
<span id="little">No Special Event!</span>
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-
</br>
+
 +
<div class="note-title">Additional templates</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1011Fig1" src="https://static.igem.org/mediawiki/2012/2/2d/KAIST_LABNOTE101112_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align='center'>
 +
<span id="little">┗ Integrase and Excisionase template</span></div>
 +
 +
</br>
 +
 +
</div>
</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="14">
<section id="14">
<div class="date">October 14<sup>th</sup> 2012</div></br>
<div class="date">October 14<sup>th</sup> 2012</div></br>
-
<div id="content_note" >
+
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
-
<span id="little">No Special Event!</span>
+
-
</br>
+
 +
<div class="note-title">pAutoIntegrase Cloning</div>
 +
 +
<div id="content_note" >
 +
 +
<span id="little">Insert DNA – OE PCR</span>
 +
</br></br>
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1014Fig1" src="https://static.igem.org/mediawiki/2012/1/12/KAIST_LABNOTE101412_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align='center'>
 +
<span id="little">Cloning into pSB4A5 is performed with gel extracted DNA fragment.</span></div>
 +
 +
</br>
 +
 +
</div>
</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="16">
<section id="16">
<div class="date">October 16<sup>th</sup> 2012</div></br>
<div class="date">October 16<sup>th</sup> 2012</div></br>
 +
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
 +
 +
<div class="note-title">pAutoSimple inserts nested PCR and aiiA template</div>
 +
<div id="content_note" >
<div id="content_note" >
-
<span id="little">No Special Event!</span>
 
-
</br>
 
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1016Fig1" src="https://static.igem.org/mediawiki/2012/c/cb/KAIST_LABNOTE101612_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
</div>
</div>
 +
</br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
 +
<div class="note-title">pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1016Fig2" src="https://static.igem.org/mediawiki/2012/c/c4/KAIST_LABNOTE101612_Fig2.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
<span id="little">single digestion with HindIII.</span>
 +
 +
</br>
 +
</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="17">
<section id="17">
<div class="date">October 17<sup>th</sup> 2012</div></br>
<div class="date">October 17<sup>th</sup> 2012</div></br>
 +
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
 +
 +
<div class="note-title">pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation</div>
<div id="content_note" >
<div id="content_note" >
-
<span id="little">No Special Event!</span>
+
 +
<b>Results</b></br></br>
 +
 
 +
<div align="center"><img id="figure" alt="2_1017Fig1" src="https://static.igem.org/mediawiki/2012/9/9e/KAIST_LABNOTE101712_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 
</br>
</br>
 +
 +
<span id="little">sizes ok</span>
 +
 +
</br>
 +
 +
 +
</div>
 +
</br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
 +
<div class="note-title">pAuto insert DNA preparation</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1017Fig2" src="https://static.igem.org/mediawiki/2012/5/56/KAIST_LABNOTE101712_Fig2.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align='center'>
 +
<span id="little">intermediate fragment nested PCR</span></div>
 +
 +
</br>
 +
<div align="center"><img id="figure" alt="2_1017Fig3" src="https://static.igem.org/mediawiki/2012/7/79/KAIST_LABNOTE101712_Fig3.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align='center'>
 +
<span id="little">Full pAuto insert DNA construct OE PCR</span></div>
 +
 +
</br>
 +
</div>
</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="18">
<section id="18">
<div class="date">October 18<sup>th</sup> 2012</div></br>
<div class="date">October 18<sup>th</sup> 2012</div></br>
-
<div id="content_note" >
+
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
-
<span id="little">No Special Event!</span>
+
-
</br>
+
 +
<div class="note-title">pAutoIntegrase enz cut check with NotI</div>
 +
 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1018Fig1" src="https://static.igem.org/mediawiki/2012/6/66/KAIST_LABNOTE101812_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
<span id="little">Lane 3, 6, 8 seem to be cloned.</span>
 +
 +
</br>
 +
 +
</div>
</div>
 +
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="19">
<section id="19">
<div class="date">October 19<sup>th</sup> 2012</div></br>
<div class="date">October 19<sup>th</sup> 2012</div></br>
 +
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
 +
 +
<div class="note-title">pAutoIntegrase Alternative sequencing with fragments amplification</div>
 +
<div id="content_note" >
<div id="content_note" >
-
<span id="little">No Special Event!</span>
 
-
</br>
 
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1019Fig1" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE101912_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
 +
</div>
</div>
-
+
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
-
+
-
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
+
</section>
</section>
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<section id="24">
<section id="24">
<div class="date">October 24<sup>th</sup> 2012</div></br>
<div class="date">October 24<sup>th</sup> 2012</div></br>
-
<div id="content_note" >
+
</br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
-
<span id="little">No Special Event!</span>
+
-
</br>
+
 +
<div class="note-title">OE template preparation</div>
 +
 +
<div id="content_note" >
 +
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/9/96/KAIST_LABNOTE102412_Fig1.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align = 'center'>
 +
<span id="little">gp47, aiiA, LuxI, bFMO for simple and pAuto</span></div>
 +
 +
</br>
 +
<div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/5/52/KAIST_LABNOTE102412_Fig2.png"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br>
 +
<div align = 'center'>
 +
<span id="little">promoter and Inverted one</span></div>
 +
 +
</br>
 +
 +
</div>
</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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<section id="25">
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<div class="date">October 25<sup>th</sup> 2012</div></br>
<div class="date">October 25<sup>th</sup> 2012</div></br>
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<div id="content_note" >
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<div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/>　Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
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<span id="little">No Special Event!</span>
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<div class="note-title">pAuto intermediate fragment OE PCR</div>
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<b>Results</b></br></br>
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<div align="center"><img id="figure" alt="1025Fig1" src="https://static.igem.org/mediawiki/2012/f/f1/KAIST_LABNOTE102512_Fig1.png"></img></div>
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</br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
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<div class="note-title">OE PCR with new primer</div>
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<div id="content_note" >
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<b>Results</b></br></br>
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<div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/a/a9/KAIST_LABNOTE102512_Fig2.png"></img></div>
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<span id="little">pAutoSimple/inverted</span></div>
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<div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE102512_Fig3.png"></img></div>
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<span id="little">pAuto/inverted</span></div>
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</div>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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Revision as of 01:55, 27 October 2012

KAIST Korea 2012 iGEM

Notebook : Labnote-October

Labnote

October

October 1st 2012

No Special Event!
Back to the Calendar
October 2nd 2012

No Special Event!
Back to the Calendar
October 3rd 2012

No Special Event!
Back to the Calendar
October 4th 2012

No Special Event!
Back to the Calendar
October 5th 2012

No Special Event!
Back to the Calendar
October 6th 2012

No Special Event!
Back to the Calendar
October 7th 2012

No Special Event!
Back to the Calendar
October 8th 2012

No Special Event!
Back to the Calendar
October 9th 2012

No Special Event!
Back to the Calendar
October 10th 2012


　Flip Flop

LuxI and Xis OE template preparation
Results

2_1010Fig1



pAutoIntegrase cloning check and Xis template vector prep
Results

2_1010Fig2

pAutoIntegrase 1-10 – HindIII cut
 pGEM-Xis 1-2 – EcoRI cut


insert DNA preparation with OE PCR
Results

2_1010Fig3

┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)

2_1010Fig4

┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)
Back to the Calendar
October 11th 2012


　Flip Flop

Additional templates
Results

2_1011Fig1

┗ Integrase and Excisionase template

Back to the Calendar
October 12th 2012

No Special Event!
Back to the Calendar
October 13th 2012

No Special Event!
Back to the Calendar
October 14th 2012


　Flip Flop

pAutoIntegrase Cloning
Insert DNA – OE PCR

Results

2_1014Fig1

Cloning into pSB4A5 is performed with gel extracted DNA fragment.

Back to the Calendar
October 15th 2012

No Special Event!
Back to the Calendar
October 16th 2012


　Flip Flop

pAutoSimple inserts nested PCR and aiiA template
Results

2_1016Fig1



pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut
Results

2_1016Fig2

single digestion with HindIII.
Back to the Calendar
October 17th 2012


　Flip Flop

pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation
Results

2_1017Fig1

sizes ok


pAuto insert DNA preparation
Results

2_1017Fig2

intermediate fragment nested PCR

2_1017Fig3

Full pAuto insert DNA construct OE PCR

Back to the Calendar
October 18th 2012


　Flip Flop

pAutoIntegrase enz cut check with NotI
Results

2_1018Fig1

Lane 3, 6, 8 seem to be cloned.
Back to the Calendar
October 19th 2012


　Flip Flop

pAutoIntegrase Alternative sequencing with fragments amplification
Results

2_1019Fig1

Back to the Calendar
October 20th 2012

No Special Event!
Back to the Calendar
October 21st 2012

No Special Event!
Back to the Calendar
October 22nd 2012

No Special Event!
Back to the Calendar
October 23rd 2012

No Special Event!
Back to the Calendar
October 24th 2012


　Flip Flop

OE template preparation
Results

2_1024Fig1

gp47, aiiA, LuxI, bFMO for simple and pAuto

2_1024Fig1

promoter and Inverted one

Back to the Calendar
October 25th 2012

　Flip Flop

pAuto intermediate fragment OE PCR
Results

1025Fig1


OE PCR with new primer
Results

2_1025Fig2

pAutoSimple/inverted

2_1025Fig2

pAuto/inverted

Back to the Calendar
October 26th 2012

No Special Event!
Back to the Calendar
October 27th 2012

No Special Event!
Back to the Calendar
October 28th 2012

No Special Event!
Back to the Calendar
October 29th 2012

No Special Event!
Back to the Calendar
October 30th 2012

No Special Event!
Back to the Calendar
October 31st 2012

No Special Event!
Back to the Calendar


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