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Team:KAIST Korea/Notebook Labnote/2012 10
From 2012.igem.org
(Difference between revisions)
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<section id="2"> | <section id="2"> | ||
<div class="date">October 2<sup>nd</sup> 2012</div></br> | <div class="date">October 2<sup>nd</sup> 2012</div></br> | ||
| + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | ||
| + | <div class="note-title">bFMO template preparation with new primer</div> | ||
| - | + | <div id="content_note" > | |
| - | < | + | |
| - | </br> | + | <b>Results</b></br></br> |
| + | |||
| + | <div align="center"><img id="figure" alt="0818Fig1" src="https://static.igem.org/mediawiki/2012/a/a3/121002.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | <span id="little"></br>We have found that our bFMO sequence was wrong. So that we have ordered new primers with right sequence.</br></span> | ||
| + | |||
| + | |||
</div> | </div> | ||
| Line 207: | Line 216: | ||
<section id="3"> | <section id="3"> | ||
<div class="date">October 3<sup>rd</sup> 2012</div></br> | <div class="date">October 3<sup>rd</sup> 2012</div></br> | ||
| - | + | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br> | |
| - | + | <div class="note-title">Visualization of inversion from GFP to RFP</div> | |
| - | + | ||
| + | <div id="content_note" > | ||
| + | <b>Procedure</b></br></br> | ||
| + | |||
| + | <span id="little"></br>bfmo gene is constructed in the vector(pJ401, Km registance) and transformed into DH5α.</br>50λ cell from stock is inoculated in 5mL LB </br></span> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | <div class="note-title"> Visualization of inversion from GFP to RFP</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | <b>Procedure</b></br></br> | ||
| + | <span id="little">We sampled the cell every 3hr. We induced one group of samples and un-induced another group of samples as a control. We took the picture of cell with confocal microscope at absorbance 500nm for GFP expression and 600nm for RFP expression. </br> | ||
| + | </span> | ||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="0818Fig1" src="https://static.igem.org/mediawiki/2012/a/ae/121003.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </br></br> | ||
| + | <b>Discussion</b></br></br> | ||
| + | <span id="little">We obtained proper data. Un-induced sample doesn’t show measurable RFP expression throughout the culture. However, induced sample shows the increase of RFP expression. Increase of GFP level in induced sample is due to the stability of GFP and time delay before inversion. </span> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | <div class="note-title"> Insert DNA preparation with OE pcr</div> | ||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="0818Fig1" src="https://static.igem.org/mediawiki/2012/2/24/121003-1.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br> | ||
| + | <div class="note-title"> Cloning</div> | ||
| + | |||
| + | <div id="content_note" > | ||
| + | |||
| + | <b>Results</b></br></br> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="0818Fig1" src="https://static.igem.org/mediawiki/2012/8/87/121003-2.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
| + | |||
| + | <div align="center"><img id="figure" alt="0818Fig1" src="https://static.igem.org/mediawiki/2012/8/8a/121003-3.PNG"></img></div> | ||
| + | <div style="clear:both;"></div> | ||
</div> | </div> | ||
| + | |||
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a> | ||
</section> | </section> | ||
Revision as of 01:11, 27 October 2012
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
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Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-October
Labnote
OctoberOctober 1st 2012
No Special Event!
Back to the Calendar
Back to the CalendarOctober 2nd 2012
Flip FlopbFMO template preparation with new primer
Results
We have found that our bFMO sequence was wrong. So that we have ordered new primers with right sequence.
Back to the Calendar
October 3rd 2012
Flip FlopVisualization of inversion from GFP to RFP
Procedure
bfmo gene is constructed in the vector(pJ401, Km registance) and transformed into DH5α.50λ cell from stock is inoculated in 5mL LB
Visualization of inversion from GFP to RFP
Procedure
We sampled the cell every 3hr. We induced one group of samples and un-induced another group of samples as a control. We took the picture of cell with confocal microscope at absorbance 500nm for GFP expression and 600nm for RFP expression.
Results
Discussion
We obtained proper data. Un-induced sample doesn’t show measurable RFP expression throughout the culture. However, induced sample shows the increase of RFP expression. Increase of GFP level in induced sample is due to the stability of GFP and time delay before inversion.
Insert DNA preparation with OE pcr
Results
Cloning
Results
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October 4th 2012
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