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Team:UC Chile/Cyanolux/Results short
From 2012.igem.org
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We built two integrative plasmid backbones for Synechocystis PCC. 6803. | We built two integrative plasmid backbones for Synechocystis PCC. 6803. | ||
| - | - | + | [File:UC_Chile-IntKplasmidResult.jpg| 480px | left] |
| + | [File:UC_Chile-IntSplasmidResult.jpg| 480px | right] | ||
<h2>Constructs for Synechocystis</h2> | <h2>Constructs for Synechocystis</h2> | ||
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Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i> (LuxAB & LuxCDEG) | Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i> (LuxAB & LuxCDEG) | ||
| - | - | + | [File:UC_Chile-IntKfullplasmidResult.jpg| 480px | left] |
| + | [File:UC_Chile-IntSfullplasmidResult.jpg| 480px | right] | ||
<h1>Characterization in Synechocystis</h1> | <h1>Characterization in Synechocystis</h1> | ||
Revision as of 02:00, 26 October 2012
Contents |
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
[File:UC_Chile-IntKplasmidResult.jpg| 480px | left] [File:UC_Chile-IntSplasmidResult.jpg| 480px | right]
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)
[File:UC_Chile-IntKfullplasmidResult.jpg| 480px | left] [File:UC_Chile-IntSfullplasmidResult.jpg| 480px | right]
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
-Image of Syne plates with colonies-
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
-image-
Experimental Highlights
