From 2012.igem.org
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Revision as of 00:39, 26 October 2012
Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
-Image of constructs- And links
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)
- Image of full construts-
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
-Image of Syne plates with colonies-
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
-image-
Experimental Highlights