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Team:Columbia-Cooper-NYC/Making Solid Media
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Sterile filter solution with: | Sterile filter solution with: | ||
<UL> | <UL> | ||
| - | <LI> | + | <LI>2 g of Na2S2O3 |
<LI>10 mL of H2O | <LI>10 mL of H2O | ||
</UL> | </UL> | ||
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<BLOCKQUOTE> | <BLOCKQUOTE> | ||
Autoclave solution of: | Autoclave solution of: | ||
| - | <UL>500 mL deionized | + | <UL>500 mL deionized water |
<LI>27 g (NH4)2SO4 | <LI>27 g (NH4)2SO4 | ||
<LI>0.9 g KCl | <LI>0.9 g KCl | ||
Revision as of 03:49, 4 October 2012
Solid Media Protocol
Solution A
Sterile filter solution with:
- 2 g of Na2S2O3
- 10 mL of H2O
Solution B
Adjust pH to 2.0 with sulfuric acid (H2SO4) and sterile filter solution with:
- 2 g Fe(II)SO4
- 10 mL of H2O
Solution C
Autoclave solution of:500 mL deionized water
- 27 g (NH4)2SO4
- 0.9 g KCl
- 1.605 g leucine
- 4.5 g MgSO4
- 0.02 g HK2PO4
- 0.485 g diaminopimelic acid
- 10 g Fe(II)SO4 (at the end)
Solution D
Autoclave solution containing:
- 6 g Agarose
- 480 mL distilled water
- Mix A & D, and B & C
- Mix together AD and BC
- Pour into petri dishes and allow to harden
Credit for protocol goes to Jason Candreva, Research Associate, Columbia University
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