Team:Columbia-Cooper-NYC/Making Solid Media
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== Solid Media Protocol == | == Solid Media Protocol == | ||
+ | '''Solution A''' | ||
+ | <BLOCKQUOTE> | ||
+ | Sterile filter solution with: | ||
+ | <UL> | ||
+ | <LI>2g of Na2S2O3 | ||
+ | <LI>10 mL of H2O | ||
+ | </UL> | ||
+ | </BLOCKQUOTE> | ||
+ | '''Solution B''' | ||
+ | <BLOCKQUOTE> | ||
+ | Adjust pH to 2.0 with sulfuric acid (H2SO4) and sterile filter solution with: | ||
+ | <UL> | ||
+ | <LI>Fe(II)SO4 | ||
+ | <LI>10 mL of H2O | ||
+ | </UL> | ||
+ | </BLOCKQUOTE> | ||
+ | '''Solution C''' | ||
+ | <BLOCKQUOTE> | ||
+ | Autoclave solution of: | ||
+ | <UL>500 mL deionized | ||
+ | <LI>27 g (NH4)2SO4 | ||
+ | <LI>0.9 g KCl | ||
+ | <LI>1.605 g leucine | ||
+ | <LI>4.5 g MgSO4 | ||
+ | <LI>0.02 g HK2PO4 | ||
+ | <LI>0.485 g diaminopimelic acid | ||
+ | <LI>10 g Fe(II)SO4 (at the end) | ||
+ | </UL> | ||
+ | </BLOCKQUOTE> | ||
+ | |||
+ | '''Solution D''' | ||
+ | <BLOCKQUOTE> | ||
+ | Autoclave solution containing: | ||
+ | <UL> | ||
+ | <LI>6 g Agarose | ||
+ | <LI>480 mL distilled water | ||
+ | </UL> | ||
+ | </BLOCKQUOTE> | ||
+ | |||
+ | <OL> | ||
+ | <LI>Mix A & D, and B & C | ||
+ | <LI>Mix together AD and BC | ||
+ | <LI>Pour into petri dishes and allow to harden | ||
+ | </OL> | ||
<i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i> | <i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i> | ||
Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] | Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] |
Revision as of 03:47, 4 October 2012
Solid Media Protocol
Solution A
Sterile filter solution with:
- 2g of Na2S2O3
- 10 mL of H2O
Solution B
Adjust pH to 2.0 with sulfuric acid (H2SO4) and sterile filter solution with:
- Fe(II)SO4
- 10 mL of H2O
Solution C
Autoclave solution of:500 mL deionized
- 27 g (NH4)2SO4
- 0.9 g KCl
- 1.605 g leucine
- 4.5 g MgSO4
- 0.02 g HK2PO4
- 0.485 g diaminopimelic acid
- 10 g Fe(II)SO4 (at the end)
Solution D
Autoclave solution containing:
- 6 g Agarose
- 480 mL distilled water
- Mix A & D, and B & C
- Mix together AD and BC
- Pour into petri dishes and allow to harden
Credit for protocol goes to Jason Candreva, Research Associate, Columbia University
Return to Protocols Page