Team:UCSF/Protocols2

To extract pigments, samples were taken from each culture and centrifuged to pellet the cells. For most experiments a 2ml culture was sufficient and this cell pellet was resuspended in the desired solvent. As seen in our data page, several solvents were tested but our final analysis was done with an ethanol extraction. After addition of the solvent (~300ul) the sample was vortexed and then centrifuged again to remove cell precipitate. The resulting organic phase was used for analysis.

Auxotroph System Protocols

Monoculture of Auxotroph Strains to determine growth rate in presence of amino acids

Co-culturing of Auxotroph Strains

Toxin/Antitoxin System Protocols

Inducing for Protein Production

Toxin-Antitoxin Exchange Protocol

@@ Line 21: / Line 21: @@
 <ul>
 <regulartext>
-<li>Growing Cultures Anaerobically</li>
 <li>Extracting Pigments</li>
+<regulartext> To extract pigments, samples were taken from each culture and centrifuged to pellet the cells. For most experiments a 2ml culture was sufficient and this cell pellet was resuspended in the desired solvent. As seen in our data page, several solvents were tested but our final analysis was done with an ethanol extraction. After addition of the solvent (~300ul) the sample was vortexed and then centrifuged again to remove cell precipitate. The resulting organic phase was used for analysis.
 </ul>