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Team:Arizona State/Magainin
From 2012.igem.org
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| - | Magainins are a class of proteins found in the skin of Xenopus laevis</html><sup>[http://en.wikipedia.org/wiki/Xenopus_laevis]</sup><html> - the African clawed frog - that contain antimicrobial properties. Both Magainin 1 and 2 are closely related peptides of 23 amino acids each and only differ by two amino acid substitutions. These two peptides have broad spectrum antimicrobial activity, including gram-positive and gram-negative bacteria, viruses, protozoa, yeasts and fungi, and are hemolytic and cytotoxic to cancer cells. | + | Magainins are a class of proteins found in the skin of Xenopus laevis</html><sup>[http://en.wikipedia.org/wiki/Xenopus_laevis]</sup><html> - the African clawed frog - that contain antimicrobial properties. Both Magainin 1 and 2 are closely related peptides of 23 amino acids each and only differ by two amino acid substitutions. These two peptides have broad spectrum antimicrobial activity, including gram-positive and gram-negative bacteria, viruses, protozoa, yeasts and fungi, and are hemolytic and cytotoxic to cancer cells. |
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Revision as of 04:45, 3 October 2012
Magainin-Split Beta-Galactosidase Biosensor
Overview
Current methods of water-borne pathogen detection require expensive equipment that often isn't modular, high throughput, or accessible to developing countries. One of the projects this year's ASU iGEM undertook was building a split-enzyme engineered fusion protein consisting of the Magainin peptide and the split alpha and omega fragments of beta-galactosidase.
Magainin
Magainins are a class of proteins found in the skin of Xenopus laevis[http://en.wikipedia.org/wiki/Xenopus_laevis] - the African clawed frog - that contain antimicrobial properties. Both Magainin 1 and 2 are closely related peptides of 23 amino acids each and only differ by two amino acid substitutions. These two peptides have broad spectrum antimicrobial activity, including gram-positive and gram-negative bacteria, viruses, protozoa, yeasts and fungi, and are hemolytic and cytotoxic to cancer cells.
