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Team:Columbia-Cooper-NYC/Plates
From 2012.igem.org
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# Apply 500µl of 1000x stock antibiotics to make 1x solution | # Apply 500µl of 1000x stock antibiotics to make 1x solution | ||
# Pour solution into plates | # Pour solution into plates | ||
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| + | Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] | ||
Revision as of 02:25, 3 October 2012
Protocol for Creating Plates
- The following is the protocol for creating plates to grow bacteria on:
- Prepare 1L bottle
- For 500mL, add 7.5g of agar and 12.5g of LB
- Pour deionized water until water level reaches 500mL
- Cover bottle with foil and tape with autoclave tape
- Autoclave on slow for 20 minutes
- After autoclaving, let bottle stand cool until touch
- Apply 500µl of 1000x stock antibiotics to make 1x solution
- Pour solution into plates
Return to Protocols Page
