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Team:Arizona State/Notebook
From 2012.igem.org
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** Grew controls to test for contamination | ** Grew controls to test for contamination | ||
*** Both Seed colonies grew, no contamination present | *** Both Seed colonies grew, no contamination present | ||
| + | |||
| + | ==June 14== | ||
| + | * Competent cell prep | ||
| + | ** Prepared CaCl2 buffer solution and CaCl2 glycerol buffer solution | ||
| + | ** Grew seed colony in 400mL LB no amp | ||
| + | |||
| + | ==June 15== | ||
| + | * Competent cell prep | ||
| + | ** Centrifuged falcon test tubes containing liquid colonies | ||
| + | ** Resuspended in CaCl2 buffer solution and incubated for 15 mins | ||
| + | ** Centrifuged and resuspended in CaCl2 glycerol buffer solution | ||
| + | ** Chilled overnight | ||
| + | |||
| + | ==June 16== | ||
| + | * Competent cell prep | ||
| + | ** Aliquotted 200uL into test tubes | ||
| + | ** Stored in -80C | ||
| + | |||
| + | ==June 17== | ||
| + | * Streak plated prepared competent cells on LB no amp plate | ||
| + | ** Colonies observed | ||
Revision as of 01:29, 1 October 2012
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Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.
June 07
- Transformation (LSE)
- Transformed DNA:
- lacZ (well 4:12G, I732019)
- p + lacO (well 1:6G, R0011)
- Cells: neb10beta (donated)
- Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
- Controls: puc19, no DNA (8 plates)
- Transformed DNA:
June 08
- Transformation results
- puc19: growth
- negative control: no growth
- lacZ, lacO: possible small colonies
- liquid culture in amp media (100 ug / ml):
- no growth of lacZ, lacO
- growth of puc19
June 12
- DH5a Competent Cell Prep
- Streak plated cells on LB no amp plate, let grow overnight
June 13
- Transformation (LSE)
- Transformed DNA:
- lacZ (well 4:12G, I732019)
- p + lacO (well 1:6G, R0011)
- Cells: DH5 alpha (donated)
- Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
- Controls: puc19, no DNA (8 plates)
- Transformed DNA:
- Transformation (istb4, Abhi)
- Transformed DNA:
- Cells:
- Protocol from:
- Controls:
- DH5a Chemically Competent cell prep
- Grew 2 seed colonies from streak plate in LB no amp
- Grew controls to test for contamination
- Both Seed colonies grew, no contamination present
June 14
- Competent cell prep
- Prepared CaCl2 buffer solution and CaCl2 glycerol buffer solution
- Grew seed colony in 400mL LB no amp
June 15
- Competent cell prep
- Centrifuged falcon test tubes containing liquid colonies
- Resuspended in CaCl2 buffer solution and incubated for 15 mins
- Centrifuged and resuspended in CaCl2 glycerol buffer solution
- Chilled overnight
June 16
- Competent cell prep
- Aliquotted 200uL into test tubes
- Stored in -80C
June 17
- Streak plated prepared competent cells on LB no amp plate
- Colonies observed
