Team:Arizona State/Notebook

From 2012.igem.org

(Difference between revisions)
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* DH5a Competent Cell Prep
* DH5a Competent Cell Prep
** Streak plated cells on LB no amp plate, let grow overnight
** Streak plated cells on LB no amp plate, let grow overnight
 +
 +
==June 13==
 +
* Transformation (LSE)
 +
** Transformed DNA:
 +
*** lacZ (well 4:12G, I732019)
 +
*** p + lacO (well 1:6G, R0011)
 +
** Cells: DH5 alpha (donated)
 +
** Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
 +
** Controls: puc19, no DNA (8 plates)
 +
* Transformation (istb4, Abhi)
 +
** Transformed DNA:
 +
** Cells:
 +
** Protocol from:
 +
** Controls:
 +
* DH5a Chemically Competent cell prep
 +
** Grew 2 seed colonies from streak plate in LB no amp
 +
** Grew controls to test for contamination
 +
*** Both Seed colonies grew, no contamination present

Revision as of 01:26, 1 October 2012


June 2012
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July 2012
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August 2012
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September 2012
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October 2012
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Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

  • Transformation (LSE)
    • Transformed DNA:
      • lacZ (well 4:12G, I732019)
      • p + lacO (well 1:6G, R0011)
    • Cells: neb10beta (donated)
    • Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
    • Controls: puc19, no DNA (8 plates)

June 08

  • Transformation results
    • puc19: growth
    • negative control: no growth
    • lacZ, lacO: possible small colonies
  • liquid culture in amp media (100 ug / ml):
    • no growth of lacZ, lacO
    • growth of puc19

June 12

  • DH5a Competent Cell Prep
    • Streak plated cells on LB no amp plate, let grow overnight

June 13

  • Transformation (LSE)
    • Transformed DNA:
      • lacZ (well 4:12G, I732019)
      • p + lacO (well 1:6G, R0011)
    • Cells: DH5 alpha (donated)
    • Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
    • Controls: puc19, no DNA (8 plates)
  • Transformation (istb4, Abhi)
    • Transformed DNA:
    • Cells:
    • Protocol from:
    • Controls:
  • DH5a Chemically Competent cell prep
    • Grew 2 seed colonies from streak plate in LB no amp
    • Grew controls to test for contamination
      • Both Seed colonies grew, no contamination present