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From 2012.igem.org

Revision as of 01:15, 1 October 2012 (view source) Rohit Rajan (Talk | contribs) (→June 08) ← Older edit		Revision as of 01:20, 1 October 2012 (view source) Rohit Rajan (Talk | contribs) Newer edit →
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	** no growth of lacZ, lacO		** no growth of lacZ, lacO
	** growth of puc19		** growth of puc19
		+
		+	==June 12==
		+	* DH5a Competent Cell Prep
		+	** Streak plated cells on LB no amp plate, let grow overnight

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Revision as of 01:20, 1 October 2012

June 2012 S M T W T F S           1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

July 2012 S M T W T F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

August 2012 S M T W T F S       1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

September 2012 S M T W T F S             1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

October 2012 S M T W T F S   1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Note from Dr. Haynes: Rohit, please transfer all of the Notebook entries from the OWW Wiki
http://openwetware.org/wiki/Haynes_Lab:Notebook/ASU_iGEM
To this page. Follow the format below. I added a couple of sections to help you get started.

June 07

• Transformation (LSE)
  • Transformed DNA:
    • lacZ (well 4:12G, I732019)
    • p + lacO (well 1:6G, R0011)
  • Cells: neb10beta (donated)
  • Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
  • Controls: puc19, no DNA (8 plates)

June 08

• Transformation results
  • puc19: growth
  • negative control: no growth
  • lacZ, lacO: possible small colonies
• liquid culture in amp media (100 ug / ml):
  • no growth of lacZ, lacO
  • growth of puc19

June 12

• DH5a Competent Cell Prep
  • Streak plated cells on LB no amp plate, let grow overnight