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-	With regards to our hypothetical results, our experimental fluorescence measurements were substantially faithful to our predictions. Looking at columns 5, 6, 7, and 8, our observations appear to be in line with what we have predicted in introducing the wild type PUF-PIN to constructs containing a control recognition site and a specific recognition site.	+	With regards to our hypothetical results, our experimental fluorescence measurements were substantially faithful to our predictions. Looking at columns 5, 6, 7, and 8, our observations appear to be in line with what we have predicted in introducing the wild type PUF-PIN to constructs containing a control recognition site and a specific recognition site. Columns 1 and 2 are negative fluorescence controls while Columns 3 and 4 are positive fluorescence controls measured without the influence of any sort of binding site.
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Revision as of 20:54, 29 September 2012

Results Overview

The results covered in this section are of the experiments overviewed in the Design section. Please note that not all the experiments have been completed or have data ready for disclosure. The data that is complete, that has been collected and compiled, is listed to the left for their respective experiments.

Mutant PUF Expression Gel

This is our PUF protein expression gel. We decided to test the PUF protein expression under different conditions to optimize it our further experiments. Lanes labeled 2 through 7 were expressed in a higher, E. Coli standard temperature of 37 degrees C. Lanes 8 through 13 were conducted in a lower temperature of 24 degrees C. The lower temperature, as suggested by the Zefang Wang Lab at the University of North Carolina, has been confirmed to result in more specific and definite PUF protein expression in DH5a.

The parts highlighted in red are significant expressions of the wild type PUF protein.

YFP Fluorescence Data

With regards to our hypothetical results, our experimental fluorescence measurements were substantially faithful to our predictions. Looking at columns 5, 6, 7, and 8, our observations appear to be in line with what we have predicted in introducing the wild type PUF-PIN to constructs containing a control recognition site and a specific recognition site. Columns 1 and 2 are negative fluorescence controls while Columns 3 and 4 are positive fluorescence controls measured without the influence of any sort of binding site.

As a reference to our aformentioned experimental design predictions. The theoretical results chart is shown here again.

mCherry Fluorescence Data