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<center><h2>PUF-PIN Fusion Proteins</h2></center> | <center><h2>PUF-PIN Fusion Proteins</h2></center> | ||
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<img src="https://static.igem.org/mediawiki/2012/e/e1/PUFPINwild.png"><br/> | <img src="https://static.igem.org/mediawiki/2012/e/e1/PUFPINwild.png"><br/> | ||
In designing our project we based our quantitative tests on fluorescence measured by a plate fluorescence reader. Our constructs were created in ways that best suit providing evidence for our hypothesis of the PUF-PIN fusion protein showing endonuclease activity. Generally, our results were collected from quantifying two main PUF-PIN fusion protein types, a wild type and a mutant type, in different conditions. These two fusion proteins had recognition sites that differed by one base pair.
Click on the list to the left to read about each of our constructs and why we decided to do them.
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