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Team:Slovenia/ModelingInteractiveSimulations
From 2012.igem.org
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| + | table{ border-radius: 30px; background:cyan; padding:10px 10px 10px 10px;} | ||
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<p> | <p> | ||
| - | Based on experimental data, we constructed | + | Based on experimental data, we constructed a quantitative model. Please see <a href="https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel">Quantitative and stability model</a> for details of derivation.</p> |
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| - | <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/ | + | <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/bistable_feedback_new" method="GET" onsubmit="return validateForm(this)"> |
<input type="hidden" name="check_submit" value="1"/> | <input type="hidden" name="check_submit" value="1"/> | ||
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| - | + | <table class="invisibile"> | |
| - | + | <tr class="invisibile"> | |
| - | <table class=" | + | |
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| - | <tr class=" | + | |
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| - | + | <td class="invisibile" width="700px" valign="top"> | |
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| + | <b>INPUT VECTORS DOSAGE</b> <br/> | ||
| + | <hr/> | ||
| - | < | + | <table> |
| + | <th><tr><td style="background: #0099FF;"><b><font color="white">The Switch</font></b></td><td></td></tr></th> | ||
| + | <th> | ||
| + | <tr><td>Construct</td><td>Dosage</td></tr> | ||
| + | </th> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/0/09/Svn12_qm_interactive_p0.png" width="300px"/></td><td> <input type="text" name="plasmid0" size="3" value="1" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/2/28/Svn12_qm_interactive_p1.png" width="200px"/><td> <input type="text" name="plasmid1" size="3" value="1" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/f/f0/Svn12_qm_interactive_p2.png" width="300px"/><td> <input type="text" name="plasmid2" size="3" value="1" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/7/70/Svn12_qm_interactive_p3.png" width="200px"/><td> <input type="text" name="plasmid3" size="3" value="1" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td> | ||
| + | <tr><td></td><td></td></tr> | ||
| + | <th><tr><td style="background: #0099FF;"><b><font color="white">Inducible system</font></b></td><td></td></tr></th> | ||
| + | <th> | ||
| + | <tr><td>Construct</td><td>Dosage</td></tr> | ||
| + | </th> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/c/cf/Svn12_qm_interactive_p4.png" width="200px"/><td> <input type="text" name="plasmid4" size="3" value="5" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/f/f7/Svn12_qm_interactive_p5.png" width="200px"/><td> <input type="text" name="plasmid5" size="3" value="5" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/9/96/Svn12_qm_interactive_p6.png" width="200px"/><td> <input type="text" name="plasmid6" size="3" value="5" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/9/9f/Svn12_qm_interactive_p7.png" width="200px"/><td> <input type="text" name="plasmid7" size="3" value="5" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td></td><td></td></tr> | ||
| + | <th><tr><td style="background: #0099FF;"><b><font color="white">Pristinamycin/Erithromycin ind. proteins</font></b></td><td></td></tr></th> | ||
| + | <th> | ||
| + | <tr><td>Construct</td><td>Dosage</td></tr> | ||
| + | </th> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/6/6a/Svn12_qm_interactive_p8.png" width="200px"/><td> <input type="text" name="plasmid8" size="3" value="30" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | <tr><td><img align="left" src="https://static.igem.org/mediawiki/2012/b/b1/Svn12_qm_interactive_p9.png" width="200px"/><td> <input type="text" name="plasmid9" size="3" value="30" onKeyPress="return numbersonly2(this, event)" maxlength="3"/> ng</td></tr> | ||
| + | </table> | ||
| + | <font color="blue">(Enter dosages for corresponding plasmids. <font color="black">To comply with the jetPEI® protocol, the sum of masses shall not exceed 200 ng. </font>)</font><br /><br /> | ||
| + | </td> | ||
| + | <td class="invisibile" valign="top"> | ||
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| + | <b>INDUCERS</b> <br/> | ||
<hr/> | <hr/> | ||
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<table> | <table> | ||
| - | + | <th><tr><td style="background: #0099FF;"><b><font color="white">Pristinamycin</font></b></td><td></td></tr></th> | |
<tr> | <tr> | ||
<td>Input times [min]</td> | <td>Input times [min]</td> | ||
| - | <td><input type="text" name="pc_start1" size="5" value=" | + | <td><input type="text" name="pc_start1" size="5" value="200" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> |
| - | <td><input type="text" name="pc_start2" size="5" value=" | + | <td><input type="text" name="pc_start2" size="5" value="5000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> |
<td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | <td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | ||
<td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | <td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | ||
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<tr> | <tr> | ||
<td>Durations [min]</td> | <td>Durations [min]</td> | ||
| - | <td><input type="text" name="pc_duration1" size="5" value=" | + | <td><input type="text" name="pc_duration1" size="5" value="200" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> |
| - | <td><input type="text" name="pc_duration2" size="5" value=" | + | <td><input type="text" name="pc_duration2" size="5" value="300" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> |
<td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> | <td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> | ||
<td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> | <td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td> | ||
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<table> | <table> | ||
| - | <th><tr><td>< | + | <th><tr><td style="background: #0099FF;"><b><font color="white">Erithromycin</font></b></td><td></td></tr></th> |
<tr> | <tr> | ||
<td>Input times [min]</td> | <td>Input times [min]</td> | ||
| - | <td><input type="text" name="rg_start1" size="5" value=" | + | <td><input type="text" name="rg_start1" size="5" value="1500" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> |
| - | <td><input type="text" name="rg_start2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | + | <td><input type="text" name="rg_start2" size="5" value="10000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> |
<td><input type="text" name="rg_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | <td><input type="text" name="rg_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | ||
<td><input type="text" name="rg_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | <td><input type="text" name="rg_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td> | ||
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<tr> | <tr> | ||
<td>Durations [min]</td> | <td>Durations [min]</td> | ||
| - | <td><input type="text" name="rg_duration1" size="5" value=" | + | <td><input type="text" name="rg_duration1" size="5" value="2000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> |
| - | <td><input type="text" name="rg_duration2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | + | <td><input type="text" name="rg_duration2" size="5" value="100" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> |
<td><input type="text" name="rg_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | <td><input type="text" name="rg_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | ||
<td><input type="text" name="rg_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | <td><input type="text" name="rg_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td> | ||
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<br /> | <br /> | ||
<b>SIMULATION</b> <br/> | <b>SIMULATION</b> <br/> | ||
| + | <hr /> | ||
| + | <input type="checkbox" name="noise" value="1" /> Apply measurement noise. | ||
| + | <br/> | ||
| + | <font color="blue">(Measured standard deviations will be applied in the calculations.)</font><br /> <br /> | ||
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Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br /> | Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br /> | ||
<font color="blue">(Simulation running time)</font><br /><br /> | <font color="blue">(Simulation running time)</font><br /><br /> | ||
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| - | + | <input type="submit" value="SIMULATE" /><br/> | |
| - | + | <font color="blue">(The results will be shown in a new browser window/tab after few seconds. Have fun.)</font> | |
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| - | + | </td> | |
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</tr> | </tr> | ||
</table> | </table> | ||
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</form> | </form> | ||
Revision as of 15:51, 15 October 2012
Quantitative model interactive simulations
Based on experimental data, we constructed a quantitative model. Please see Quantitative and stability model for details of derivation.
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