Team:SDU-Denmark/labwork/Protocols/gel

From 2012.igem.org

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<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td>
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<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenisis</a></td>
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<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenesis</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td>
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td>

Revision as of 21:10, 26 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenesis PCR-,gel clean-up

Gel Electrophoresis

Construct a gel - Medium size


1.      Weigh 0.8g agarose

2.      Mix with 100ml TBEx1 solution

3.      Heat in a microwave until all the agarose is dissolved and the solution is clear.

4.      Cool it down a bit before adding Ethidium Bromide (warning: toxic)

5.      Pour the solution into a gel casting mold and use a comb of desired size.

6.      Run at 100V for 40 minutes.

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