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Team:HKUST-Hong Kong/Achievement
From 2012.igem.org
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We are proud to say that within our three months of wet lab work we have achieved the following:</p> | We are proud to say that within our three months of wet lab work we have achieved the following:</p> | ||
<ol> | <ol> | ||
| - | <li>Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of <i>Bacillus subtilis</i> ( | + | <li>Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of <i>Bacillus subtilis</i> (<a href="http://partsregistry.org/Part:BBa_K733007" target="_blank">BBA_K733007</a>).</li> |
<li>Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM respectively (BBa_K733016, BBa_K733017).</li> | <li>Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM respectively (BBa_K733016, BBa_K733017).</li> | ||
<li>Successfully constructed a cell growth inhibition device for regulating BMP2 production (BBa_K733012).</li> | <li>Successfully constructed a cell growth inhibition device for regulating BMP2 production (BBa_K733012).</li> | ||
Revision as of 15:37, 26 September 2012
Achievement
Our iGEM journey has spanned the length of six months; from the day our project was first proposed in mid-March to the day we ended wet lab work in mid-September.
We are proud to say that within our three months of wet lab work we have achieved the following:
- Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of Bacillus subtilis (BBA_K733007).
- Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM respectively (BBa_K733016, BBa_K733017).
- Successfully constructed a cell growth inhibition device for regulating BMP2 production (BBa_K733012).
- Successfully characterized the low efficiency promoter pTms which was used to drive the expression of antitoxin YdcD (BBa_K733009, BBa_K733018).
- Successfully characterized a xylose-inducible promoter that was used to control the expression of BMP2 and toxin YdcE.
- Successfully characterized the cell growth inhibition device.
