Procedure:
In the main display window, the first ribbon is the sequence that the
user has inputted, and the third ribbon shows the corresponding gene annotation.
You can click any annotation that you want, and then the sequence site will
automatically jump to the fragment related to it. Between those ribbons is an
adjustable slider, which can adjust your view location by sliding to the left or
to the right. Above the sequence are the head and tail of the location that you
are viewing while the head and tail of the whole sequence are displayed in the
bottom textbox. What’s more, the textbox contains the length, product, tag of
the sequence, and links of related papers are also included sometimes.
On the left, there are two useful tools: you can search
the fragment that you want by position, product or name (Position represents the
position of its head, Product and Name stand for products and names of related
genes, both of them support fuzzy search), besides, whether you choose product
or name, a dialog box that contains names of related genes will appear; the
slider of Zoom is designed for regulating the display frame rate of the
sequence.
Procedure:
In the main display window, you can view the information of existing
biobricks, which are all localized and enable you to get rid of loading when you
are studying a biobrick. On the left is the list of the files that you have
opened.
Procedure:
On the right is the main operating area and you can choose your target
type (your experimental object) and corresponding target name, aptamer and
regulation direction. Attention should be paid to the input box named Input your
Target. You can search your target name by key words or even several letters of
it. It should be useful to you. Click GO and you will see a picture. The
designed riboswitch is in the dotted box on the top left corner. Below it is a
diagram to show how the riboswitch works. Besides, there is a list on the left
box. It records your choices that you have made in created riboswich project,
and you need only click GO next time.
Procedure:
The left textbox is for you to input DNA sequence. There are two ways
to input that: you can either input the sequence directly or open a file in
FASTA format. If you choose the former, click Direct Input, then input your
sequence, and click Go. If you choose the latter, you need only click Open Fasta
and choose your FASTA file to open. Two solutions are provided on the right——one
is solved by Tom Tuschl’s method and the other is by rational design.
Procedure:
In the main display window, you will see a network from KEGG website.
On the left box displays a list of the files you have opened. Pay attention that
you can right click the main display window to open another KEGG xml file or to
search any element in the network (choose find element).
Procedure:
There are 5 options for you to select: exhaustive single deletion,
reaction deletion, Fva analysis, Perturbation analysis and
PhPP analysis. They with FBA solve will be introduced in detail. You can
select all of them or part of them, or even nothing, then click Go. If you
select none of them, you will see a table showed basic information of each
reaction. If you select several of them, the corresponding windows will tiled in
the resulting window. If you select reaction deletion option, a selection of the
reaction you want to wipe off is required, and if there is any change, it will
be highlighted in red font.
1.FBA_solve
Left column displays names of the reactions while the
right one displays the flux amount of each reaction
2. exanstive single deletion
Calculate the flux amount of the objective reaction when
each other reaction is deleted one by one.
Left column display names of the reactions
The right one displays the flux amount of objective
reaction the user has chosen, when each other reaction is deleted one by one.
.
when rolling down the screen, you can see the
reaction-names related to lethal deletions, sub optimal deletions and super
optimaldeletions.
This graph shows how many lethal knockouts in each
reaction subsystem. The information of the reaction subsystem can be attained in
SBML
This hist graph shows the frequency of reaction which has
different quantified effect showed in the X-axis opon objective reaction.
3. reaction deletion
Middle column displays the flux amount of the wild type
while the right column displays the mutant type, which is calculated based on
the deletion of one chosen reaction.
4. FVA analysis
This analysis exams the robustness of the whole metabolic
network by calculating flux value which can be reached in reality. In other
words, this function works out the upper and lower limit of the flux of each
reaction, as you can see in the column name ‘lower
flux limit ’and ‘upper
flux limit’.
5.Perturbation analysis:
X-axis refers to the continuously increasing flux value of
the chosen reaction.
Y-axis refers to the flux value of the objective reaction
according to the variation of the flux value of chosen reaction in the X-axis
6.PhPP analysis
The value marked on the contour represents the flux value
of the objective reaction based on the variation of two chosen reactions The
color of the graph changes according to the altitude, namely, the objective flux
value.
Procedure:
You can open several file so that you can compare the difference of
these genomes. Each genome have 2 circles in this picture: the outer one is the
genes of the genome, the inner is the expression level of corresponding genes
under two different conditions. By the way, right click different sites in the
small panoramic window or left click and drag the picture in the zoom window,
you can move to anywhere you want to enlarge and see more details in the zoom
window.
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