Team:Freiburg/Notebook/Methoden

From 2012.igem.org

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==<span style="color:#000000"> First Extension PCR ==
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This step is necessary to add the the necessary restriction sites to the Direpeats.
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Pipette the following volumes into a PCR- tube:
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{|cellpadding="10" cellspacing="0" border="1"
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|'''Component'''
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|'''Volume'''
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|-
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|Water, nuclease-free
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|15,75 µl
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|-
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|DMSO
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|0,75 µl
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|-
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|dNTPs
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|0,5 µl
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|-
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|Phusion Buffer
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|5,0 µl
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|-
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|Phusion Polymerase
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|0,25 µl
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|-
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|Water, nuclease-free
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|up to 20 µl
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|-
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|'''Total vloume'''
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|'''20 µl'''
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|}
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</p><br>
</p><br>

Revision as of 17:11, 25 September 2012






First Extension PCR

This step is necessary to add the the necessary restriction sites to the Direpeats. Pipette the following volumes into a PCR- tube:

Component Volume
Water, nuclease-free 15,75 µl
DMSO 0,75 µl
dNTPs 0,5 µl
Phusion Buffer 5,0 µl
Phusion Polymerase 0,25 µl
Water, nuclease-free up to 20 µl
Total vloume 20 µl



bla

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