Team:SYSU-Software/Tutorial

From 2012.igem.org

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   <div id="content">                  <div class="entry page clear">
   <div id="content">                  <div class="entry page clear">
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         <h1 id="doctitle" class="pagetitle">Software Tutorial</h1>
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         <h1 id="doctitle" class="pagetitle">Our Project</h1>
<table class="doc-toc">
<table class="doc-toc">
<tbody>
<tbody>
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</div>
</div>
<ul id="toc-ul">
<ul id="toc-ul">
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<li class="toclevel-1"><a href="#r1"><span class="tocnumber"></span> <span class="toctext">Section I: Genome Browser</span></a></li>
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<li class="toclevel-1"><a href="#r0"><span class="tocnumber"></span> <span class="toctext"> Introduction</span></a></li>
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<li class="toclevel-1"><a href="#r2"><span class="tocnumber"></span> <span class="toctext">Riboswitch</span></a></li>
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<li class="toclevel-1"><a href="#r2">Genome Browser</a></li>
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<li class="toclevel-1"><a href="#r3"><span class="tocnumber"></span> <span class="toctext">SiRNA</span></a></li>
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<li class="toclevel-1"><a href="#r3">Biobrick</a></li>
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<li class="toclevel-1"><a href="#r4"><span class="tocnumber"></span> <span class="toctext">KEGG Network</span></a></li>
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<li class="toclevel-1"><a href="#r4">Riboswitch</a></li>
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<li class="toclevel-1"><a href="#r5"><span class="tocnumber"></span> <span class="toctext">Fba</span></a></li>
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<li class="toclevel-1"><a href="#r5">SiRNA</a></li>
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<li class="toclevel-1"><a href="#r6">Meta Network</a></li>
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<li class="toclevel-1"><a href="#r7">Simulator</a></li>
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<li class="toclevel-1"><a href="#r8">G-Circle</a></li>
</ul>
</ul>
</td>
</td>
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</table>
</table>
<div id="doc-contents">
<div id="doc-contents">
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<h2 id="r1"><a href="https://static.igem.org/mediawiki/2012/2/21/Tutorial.pdf">
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<h1><a name="_Toc336260397">Genome </a>Browser</h1>
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Download a more detailed tutorial</a></h2>
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<h2 id="r6">Genome Browser</h2>
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  stroked="f">
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Procedure: File→Open Fasta→Open xml Component</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Parts: In the main display window, the first ribbon is the sequence that the  
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 +
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 +
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 +
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4KGHuBBeBqLfXV85S+2B9D6g7sEjxgNCY9hJfyA75GDGcjNXbH6HKO7dXG0Zo+ptTVaM5vjMDL2G
 +
t0+xA17V9Un99nHmhoSgf5zB1HUc1CGU42huNxZua/KY/fJYCs9cp4uyCq4bj0W7UHhqqQfCsTHd
 +
v63BkrKiV4UdJu851o1FoWNhED3OsWM1DBEXWwJIfN0hMT0OOBwx478JZL/Ljs8I6gPMhd4kETQ4
 +
Fpdm03ibuPqKs+yAs6ezOF9xwFkrswlMz5ajgXp2QLH5r9h+yOeH2N76LWr7gXT759DfcO3gHxG/
 +
u7Yttq2fZhf6fgWbGEFvYdrcrw8Pg32kAzWn3YJI9YspILD6sQWtqx5mDYKFp7/WOY81xLMXg8OB
 +
RZmshtZN40K3b3AbIr9iCAr7pSdYr4xm4cKq/GiCJpsyp79SDl8qnJFIbClkpB6I36BcQHANVItw
 +
ZA16bW+zYPqdgQWEj8/QpGAR6M66snQo8MfeR1iPPcHkjz1BOIcn/aTXYf0Nkzua7fvT9Oyly5iC
 +
M8bvPpWrzkOyXN0IUoPPIG26GNjDDFxIYGp3TZ5sXNeOAYvfvR6z+dImxvgLPINbTc/+zjudYtZd
 +
1l7Rq4G5bO76V6+2GgTzAw96E7MjCWZU04h/rNm913TEB2gotea4SSf45a3L8LxbDQVq27WPnyeH
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UcGefiDtxlX5hKuqNh/hbRyDB0ZiFB/btWfTk64S9KxpE8fqB2r0lkRzmBeO2xJ11t8vddnmSUah
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uZsPgo55fnpsx+zXi6ceKoayxn01QFn/5aMQbHswyELHwmbzm45C5svW+Cj0/9DTfqycu5VrD3qC
 +
3iQy9PzgVv5Oxhu/Spmu79CPBgf2PfOFKxBf0fe0NQPL3H+sAYo0/HvG927AX+68/hsAAP//AwBQ
 +
SwMEFAAGAAgAAAAhAIu+tnKiBAAA0hMAABkAAABkcnMvZGlhZ3JhbXMvZHJhd2luZzEueG1s7FjN
 +
jts2EL4X6DsQuhaKJVmSZWO9wUqJgwBpslhv0F5pibKFUqRK0l57iz5Bj32PvkHfpn2PDn9kO94N
 +
NmnRpgfvgSsOZ0bf/HBmrIvn25aiDRGy4Wzqhc8CDxFW8qphy6n3/nbmZx6SCrMKU87I1NsR6T2/
 +
/Pqri0p2k0rgO2BEoIPJSbVsp95KqW4yGMhyRVosn/GOMDituWixgq1YDpxQSwdREKSDqsFLgVuv
 +
VyK7B0raphRc8lo9K3k74HXdlKRXo5Vkp0rwAxVP42hxw7xLY5bsbgUh9pltXolu3l0Luy3fbq4F
 +
aqqpB45iuAWPeIP9kWMFwkC750R2aRU5dtmhlleEvgZdP+VRkQ3zNPWviqLw4/Rl6GfJuPCLKM/i
 +
JM1fpFn8s0PHNp8KR/MdsBykpDEHT7a1aC8v8AQ8irZTL4pCCPZOmwZSeEK2CpVAD4dRGiahh0o4
 +
i7NkNEo0w+CgoBNSvSK8Rfph6kHsN4Jrb+IJ3ryRynL3XJoMIa9mDaVIcPVdo1bzFe7Al4GRWUqQ
 +
MVwSdVz2ZCmWi4IKtMEUcMyyMH9h2Fdr8g4sMGQHXWJ1SqLr9pSEabfCp0S5whWxypIwCA4Kv+WV
 +
JYdDIBs6uMCBMu5YymPQmeHSlP8c+FgjtEEET3wucG01iH8Z5PFHkCeO/ojLgbTPGNowhHXpClMo
 +
DFoGyRJTYi6sTcM+9bR9lOmVcZ2K9tRSSF2TUrks5GtFxHxV3aEFXYsbDLo0SlBdNTrdI+Nt2Iip
 +
l5gTOHosrz/IA63Bednkoc2t4Ufs3GMwiXYEzxQae6F1yZFqR6FyactuSA2FCu6vC2Xprg+gBHzg
 +
IlgXeu0NBwEtWYMv9rJDmwZPyDoRLW2h7eWjT5LfC5n3c6b28m3DuHA6oKOQ/f2nKnTAa8vvaq5z
 +
gPaF2ua82mmVC/gPZVt25ayBCvUGS3WNBXQJcAH0Pojhiot7D90JDN1H/rjGgniIvmZQe2K4x6mH
 +
lNmEaRBEENzjzeJ4w9ZtwaE8QbWEt9nHaASvwayEd0BtVKLfFMqEQgNk/GqteN24Smnx6gMq1VyH
 +
FGKEJ51ZwBC6gVdopXQJTduorEh9ixfze0jB4RCS0PiMsnlXaiHAcl0qm2Jjk3d90A8MuQ3/MWuf
 +
G0A7nF7VpjYf832QtV1pOOBWujYjNAKhYZubSZj/fg7uAagh3FEP/UCEHj30s8HKaWN6g9ns5D7k
 +
MGpU/A7iAuED4tSbmT+XBvJYjGIFhUDtOlLjEvpKgWmzEI0tiQQfnXzTMp9ge1DKk4NSOuWAXqNR
 +
l1AoiO584AJYDZGwSifTzd7A+5VfvNXBgUTTpej/ZfCfv/3yx++/PmXwwShjKGSeKTT9lbLX6/uT
 +
+SGGhglX5eEEkcXBcAQnJwPEsRZXx/oB7Gg2gj5fzEZZ7gdRHvlxGL3083Ey9vNZehWGV8FsPBv+
 +
67NRGI6TURI/Ztx5PIL68vlTxnk8+hsT6fg8HkGXsL3LTVOmPp3HI/OD+zwe6ZnjPB59sfHoHXzu
 +
QTOYz/B5SDKfktT2ZEgK42GWxvAb4p9NSW5cst+pzMZ9Vrv8CwAA//8DAFBLAQItABQABgAIAAAA
 +
IQBgJjcoXAEAAHYEAAATAAAAAAAAAAAAAAAAAAAAAABbQ29udGVudF9UeXBlc10ueG1sUEsBAi0A
 +
FAAGAAgAAAAhADj9If/WAAAAlAEAAAsAAAAAAAAAAAAAAAAAjQEAAF9yZWxzLy5yZWxzUEsBAi0A
 +
FAAGAAgAAAAhAHM8L9TOBwAAgSEAABYAAAAAAAAAAAAAAAAAjAIAAGRycy9kaWFncmFtcy9kYXRh
 +
MS54bWxQSwECLQAUAAYACAAAACEAbYK5dycBAABnAgAADgAAAAAAAAAAAAAAAACOCgAAZHJzL2Uy
 +
b0RvYy54bWxQSwECLQAUAAYACAAAACEA0jPc+R0BAABmAwAAGQAAAAAAAAAAAAAAAADhCwAAZHJz
 +
L19yZWxzL2Uyb0RvYy54bWwucmVsc1BLAQItABQABgAIAAAAIQBKTh0O3AAAAAkBAAAPAAAAAAAA
 +
AAAAAAAAADUNAABkcnMvZG93bnJldi54bWxQSwECLQAUAAYACAAAACEAA8CiPBEEAADfQQAAGAAA
 +
AAAAAAAAAAAAAAA+DgAAZHJzL2RpYWdyYW1zL2NvbG9yczEueG1sUEsBAi0AFAAGAAgAAAAhAGub
 +
lhQYBAAADVEAABwAAAAAAAAAAAAAAAAAhRIAAGRycy9kaWFncmFtcy9xdWlja1N0eWxlMS54bWxQ
 +
SwECLQAUAAYACAAAACEAQ7iJ78gGAABDJQAAGAAAAAAAAAAAAAAAAADXFgAAZHJzL2RpYWdyYW1z
 +
L2xheW91dDEueG1sUEsBAi0AFAAGAAgAAAAhAIu+tnKiBAAA0hMAABkAAAAAAAAAAAAAAAAA1R0A
 +
AGRycy9kaWFncmFtcy9kcmF3aW5nMS54bWxQSwUGAAAAAAoACgCbAgAAriIAAAAA
 +
">
 +
  <v:imagedata src="file:///I:\msohtmlclip1\01\clip_image001.png" o:title=""/>
 +
  <o:lock v:ext="edit" aspectratio="f"/>
 +
  <w:wrap type="topAndBottom" xmlns:w="urn:schemas-microsoft-com:office:word"/>
 +
</v:shape></o:wrapblock><br clear="ALL">
 +
<span lang="EN-US">Procedure: <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/0/03/Image0001.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/f/f5/Image002.jpg"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/1/1e/Image0003.jpg" style="width: 650px;"><br clear="ALL">
 +
<span lang="EN-US">
 +
<v:shape id="图片_x0020_8" o:spid="_x0000_i1046"
 +
type="#_x0000_t75">
 +
<v:imagedata src="image003.jpg" o:title=""/>
 +
</v:shape>In the main display window, the first ribbon is the sequence that the  
user has inputted, and the third ribbon shows the corresponding gene annotation.  
user has inputted, and the third ribbon shows the corresponding gene annotation.  
You can click any annotation that you want, and then the sequence site will  
You can click any annotation that you want, and then the sequence site will  
Line 99: Line 304:
are viewing while the head and tail of the whole sequence are displayed in the  
are viewing while the head and tail of the whole sequence are displayed in the  
bottom textbox. What’s more, the textbox contains the length, product, tag of  
bottom textbox. What’s more, the textbox contains the length, product, tag of  
-
the sequence, and links of related papers are also included sometimes. </p>
+
the sequence, and links of related papers are also included sometimes. <o:p></o:p>
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;On the left, there are two useful tools: you can search the fragment that you want by position or name (Position represents the position of its head and Name stands for its tag or key word of its gene annotation); the slider of Zoom is designed for regulating the display frame rate of the sequence.
+
</span></p>
-
</p></div>
+
<p><span lang="EN-US">On the left, there are two useful tools: you can search  
-
<h2 id="r0">Biobrick</h2>
+
the fragment that you want by position, product or name (Position represents the  
-
<div>
+
position of its head, Product and Name stand for products and names of related
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Procedure: Open your biobrick file(in xml format)</p>
+
genes, both of them support fuzzy search), besides, whether you choose product
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Component Parts: In the main display window, you can view information of existing biobricks. On the left is the list of the files that you have opened.
+
or name, a dialog box that contains names of related genes will appear; the  
-
</p>
+
slider of Zoom is designed for regulating the display frame rate of the  
-
</div>
+
sequence. <o:p></o:p></span></p>
-
<h2 id="r2">Riboswitch</h2>
+
<h1><a name="_Toc336260391"><span lang="EN-US">Biobrick</span></a><span lang="EN-US"><o:p></o:p></span></h1>
-
<div>
+
<p><o:wrapblock>
-
<p>Procedure: choose a target type→choose a target name→choose an aptamer→choose a regulation direction (up regulation or down regulation) →GO
+
<v:shape id="图示_x0020_4" o:spid="_x0000_s1027"
-
</p><p>Component Parts: On the right is the main operating area and you can choose your target type (your experimental object) and corresponding target name, aptamer and regulation direction. Attention should be paid to the input box named Input your Target. You can search your target name by key words or even several letters of it. It should be useful to you. Click GO and you will see a picture. The designed riboswitch is in the dotted box on the top left corner. Below it is a diagram to show how the riboswitch works. Besides, there is a list on the left box. It records your choices that you have made in created riboswich project, and you need only click Go next time.
+
  type="#_x0000_t75" o:gfxdata="UEsDBBQABgAIAAAAIQBgJjcoXAEAAHYEAAATAAAAW0NvbnRlbnRfVHlwZXNdLnhtbLSUy07DMBRE
-
</p></div>
+
90j8Q+QtStx2gRBq0kVTVggQlA+4sm9Sq34E20mbv8dJU1U8Ctl06cfMHF2PPF/slYwatE4YnZJp
-
<h2 id="r3">SiRNA</h2>
+
MiERama40GVK3tcP8R2JnAfNQRqNKWnRkUV2fTVftxW6KKi1S8nG++qeUsc2qMAlpkIdTgpjFfiw
-
<div>
+
tCWtgG2hRDqbTG4pM9qj9rHvPEg2z7GAWvpotQ/bBxKL0pFoebjYZaUEqkoKBj6Q0kbzbynxkJAE
-
<p>Procedure: Input your DNA sequence</p>
+
ZX/HbUTlbgIGob8mdCfnAwbdcxiNFRyjF7D+CVTAoNw6ijOTG5b87dFBKhebohAMk9y6Va86Mp3z
-
<p>Component Parts: The left textbox is for you to input DNA sequence. There are two ways to input that: you can either input the sequence directly or open a file in FASTA format. If you choose the former, click Direct Input, then input your sequence, and click Go. If you choose the latter, you need only click Open Fasta and choose your FASTA file to open. Two solutions are provided on the right——one is solved by Tom Tuschl’s method and the other is by rational design.  
+
5gJKC8pRDh6mIzK+jnvI44bVKgw54RZ24TWVTAbjPNiOZpDQmtpfgOKxNx7N8VELtn3zrcQLsPS+
-
</p></div>
+
o1GYkca6C2Ase+PRHMPDjgE5tfBnGw71+DfV7LTFZkQbT1mh8XmQvWJzdKf9r5F9AgAA//8DAFBL
-
<h2 id="r4">KEGG Network</h2>
+
AwQUAAYACAAAACEAOP0h/9YAAACUAQAACwAAAF9yZWxzLy5yZWxzpJDBasMwDIbvg72D0X1xmsMY
-
<div>
+
o04vo9Br6R7A2IpjGltGMtn69jODwTJ621G/0PeJf3/4TItakSVSNrDrelCYHfmYg4H3y/HpBZRU
-
<p>Procedure: open a file (in xml format)</p>
+
m71dKKOBGwocxseH/RkXW9uRzLGIapQsBuZay6vW4mZMVjoqmNtmIk62tpGDLtZdbUA99P2z5t8M
-
<p>Component Parts: In the main display window, you will see a network from KEGG website. On the left box displays a list of the files you have opened. Pay attention that you can right click the main display window to open another KEGG xml file or to search any element in the network (choose find element).
+
GDdMdfIG+OQHUJdbaeY/7BQdk9BUO0dJ0zRFd4+qPX3kM66NYjlgNeBZvkPGtWvPgb7v3f3TG9iW
-
</p></div>
+
Oboj24Rv5LZ+HKhlP3q96XL8AgAA//8DAFBLAwQUAAYACAAAACEAmwlY+AUGAACxEgAAFgAAAGRy
-
<h2 id="r5">Fba</h2>
+
cy9kaWFncmFtcy9kYXRhMS54bWzcWM1u3DYQvhfoOxA6tSiYlbSUVlpkHei3NeDGQeykh6IouBJ3
-
<div>
+
Vy31E1Ib2wly7qHHAj31EYq+Qd+mzXN0SHHXa8duNi7gFvVB1g6pjzPDmW+GfPjovOboJROyapuZ
-
<p>Procedure: Open a file (in xml format)→select your options→Go</p>
+
5TywLcSaoi2rZjmznp3mOLCQ7GlTUt42bGZdMGk9Ovj4o4flsp6WtKdftiXjCFAaOQXZzFr1fTcd
-
<p>Component Parts: There are 5 options for you to select: exhaustive single deletion, reaction deletion, fva solve, preturbation analysis and PHPP analysis. You can select all of them or part of them, or even nothing, then click Go. If you select none of them, you will see a table showed basic information of each reaction. If you select several of them, the corresponding windows will tiled in the resulting window. If you select reaction deletion option, a selection of the reaction you want to wipe off is required, and if there is any change, it will be highlighted in red font.
+
jWSxYjWVD9qONTC6aEVNe/gplqNS0DPAr/nItW1/VFZ0KWhtGRB6B4iaVo11oHXq+iPZb15RrbQ7
-
</p></div>
+
LGfWazex7SideDjIwwiT3LdxmIYEp/HE9byJ64/j8I2F+osOrCzbYgMnTliPeHsKcoWzFs20rgrR
-
 
+
ynbRPyjaetQuFlXBzD8qemWUNwpGnF60637UibZgUjoWgCS0VxhGZKEX8sNhX6yr4nvZX3A2klXd
 +
cUYUjkEeJBYq7gBctLwVckSLgjW9862rUAyskVmoW/FSQJhYo8HDsnsi4HWkgqG7yetx5mZulDk4
 +
I/EYEycPcBw4Cc78iRP6ziQOwvzNFVfrJU5n1tdvf/7h7S+/frO71MFDOj1fiFr9B7ejc1BlHELI
 +
XswsW02kU3beowLkbkA8JxyDETBGQs8NQzVhdAnQCdl/ztoaqZeZJdp1Uz5lRQ/a0Cl9qaOITpcl
 +
amgNIUHL7yy0qDmE50vKkWPDn0E0kwF7g6kgIKbLvOIcibb/qupXJyuqQsvW+Eu5wZeoa+VGLMVy
 +
nnCBYAXQOg+cONXTV2t2DPZqsTFU0v66iK/r6yLKuxW9LpQrWrIBzFN2DJ4DQMjlQeyMd8wzSmnn
 +
LaU2zSgd6FlKcu+Kh0rDOyuud2/YifvXnNyiuWfkEEfXXQ6ibcTwqkFUsbPjA9Oob5AsKGdALJuA
 +
3ISe2hneqGfTqlAcEmCQsMUCgt1EIRAVEyer8gzN+Vo8pYCltAToslLJ4Wpvww9Ifk+PwNBNcX3F
 +
mwoBMNT6Og6H2BrfYudWBx1oO+ppdlFMM3AO0AydztvyQlMPGCj7E02HSt6pB0yExxOBuPYTa/Cz
 +
E62FbHmlc1JNkBdym2pQjsr2DCiayh6EMyvXfya/r3zGaQ8boMrEghaQzwnl1VxUQygyujPyWd1g
 +
RoeBQl4bKKQBBz2VNv3BMRRKBCVDoHnVAmLxPVpUnKFPYDlVpIcK+qmiMHABPPV3rCmfUEGfbq19
 +
tcLJYwtR3h/9q9b/+duPf/z+0/usv1RfmwTbp3dbGXhrTckS1wfa97Hr5wkmdgKV3IkCHLl+NvGz
 +
zJ3k3raSd1ScCtpIKATnjW4EoiTKoEK72PdjaATcMMZxrB5JmAROSKI0t6+UJMgapYupder1/SF4
 +
aZcJwpu3ZUjIvayOJqnjBWBm4jkxJkHu4ND2Y+wFwdiNbGITJ9paLav5/8Pq2PYTD3YUR8QNBqsj
 +
Yoc4DwI7zhw7mcTu1upOQHNqWj/dtilBJGVbHKb7d4Dqo8e65Ksn8JwSaIZJGqBCxWc6NEF6pFu8
 +
51RAVVQy4EcTLDBYvWJfQN/MmRx4j51TaC7Ujt/0uRFCs3kl2m7PgtybOEE48XE8TiEL4oDgIBpD
 +
o+WnQTLO4zzM9/bMfl2adsTgmQba6h3HHM2hXVEy6HK33josoQ17x33O37hvvuac9VlD51DNTCPy
 +
oQ67p/Q0+6LLp1oSyGX3fefcsR/dICmKDzmmlEzqo8TrPbcO4I8FVHVV0eHTzfuGHfXSe/LqhlsG
 +
Mt2LlUxSgJN2HBM6aTixSY5TkqSYRJGLozDxcOr4Se6Nk3gSAA+bA5mKquMFdDrGTXuavXXTntly
 +
m5tgdWXu3Q9/N3oA0jWCzsrHxI9dTDIoR2HmJdgmKSFpFGeEXB5Jr3tgz/Ps1gN7Mun9eiCcjDM3
 +
zwKcjeFkTjKSAZ05Pk6DnBCoygnxJldiAHqdK2GwX3nYOuE/EAaaOXbpYr40wXG2auFQP9A/HGNN
 +
a64OtGtRvXMpcuMlhLlZUVcQwfZeBRBld3lXkwGgua6R3T+ARWK4WRGHpWehumqeM2ic73z7A4ZD
 +
TwvWmgK58YD21/ai6eAvAAAA//8DAFBLAwQUAAYACAAAACEACifWuSgBAABnAgAADgAAAGRycy9l
 +
Mm9Eb2MueG1spJLLSgMxFIb3gu8Qsrdp04vT0JluitCVG32AmJzMBHIzSR19e08vSF0Jdfefc+Dj
 +
408220/vyAfkYmNo6WwypQSCitqGvqWvL08PDSWlyqCliwFa+gWFbrv7u82YBPA4RKchE4SEIsbU
 +
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">
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  <v:imagedata src="file:///I:\msohtmlclip1\01\clip_image004.png" o:title=""/>
 +
  <o:lock v:ext="edit" aspectratio="f"/>
 +
  <w:wrap type="topAndBottom" xmlns:w="urn:schemas-microsoft-com:office:word"/>
 +
</v:shape></o:wrapblock><br clear="ALL">
 +
<span lang="EN-US">Procedure: <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/6/6a/Image0004.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/b/b7/Image005.jpg" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_19"
 +
o:spid="_x0000_i1045" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image005.jpg" o:title=""/>
 +
</v:shape>In the main display window, you can view the information of existing  
 +
biobricks, which are all localized and enable you to get rid of loading when you
 +
are studying a biobrick. On the left is the list of the files that you have  
 +
opened. <o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<h1><a name="_Toc336260392"><span lang="EN-US">Riboswitch</span></a><span lang="EN-US"><o:p></o:p></span></h1>
 +
<p><span lang="EN-US">Procedure: <span>
 +
<v:shape id="图示_x0020_2" o:spid="_x0000_i1044"
 +
type="#_x0000_t75"
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">
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<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image006.png" o:title=""
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  croptop="-7957f" cropbottom="-1952f"/>
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</v:shape><v:shape id="图片_x0020_17" o:spid="_x0000_i1043" type="#_x0000_t75">
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</v:shape></span><o:p></o:p>
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</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/3/3e/Image006.png" style="width: 630px;></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/5/5c/Image0007.jpg" style="width: 650px;></p>
 +
<p>
 +
<v:shape id="图片_x0020_12"
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o:spid="_x0000_i1042" type="#_x0000_t75">
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<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image008.jpg" o:title=""/>
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</v:shape>
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<img src="https://static.igem.org/mediawiki/igem.org/0/06/Image008.jpg" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_12"
 +
o:spid="_x0000_i1042" type="#_x0000_t75">
 +
</v:shape>
 +
On the right is the main operating area and you can choose your target  
 +
type (your experimental object) and corresponding target name, aptamer and  
 +
regulation direction. Attention should be paid to the input box named Input your  
 +
Target. You can search your target name by key words or even several letters of  
 +
it. It should be useful to you. Click GO and you will see a picture. The  
 +
designed riboswitch is in the dotted box on the top left corner. Below it is a  
 +
diagram to show how the riboswitch works. Besides, there is a list on the left  
 +
box. It records your choices that you have made in created riboswich project,  
 +
and you need only click GO next time.<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<h1><a name="_Toc336260393"><span lang="EN-US">SiRNA</span></a><span lang="EN-US"><o:p></o:p></span></h1>
 +
<p><o:wrapblock>
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">
 +
  <v:imagedata src="file:///I:\msohtmlclip1\01\clip_image009.png" o:title=""/>
 +
  <o:lock v:ext="edit" aspectratio="f"/>
 +
  <w:wrap type="topAndBottom" xmlns:w="urn:schemas-microsoft-com:office:word"/>
 +
</v:shape></o:wrapblock><br clear="ALL">
 +
<span lang="EN-US">Procedure: <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/c/c1/Image009.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/5/5d/Image0010.jpg" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_5"
 +
o:spid="_x0000_i1041" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image010.jpg" o:title=""/>
 +
</v:shape>The left textbox is for you to input DNA sequence. There are two ways  
 +
to input that: you can either input the sequence directly or open a file in  
 +
FASTA format. If you choose the former, click Direct Input, then input your  
 +
sequence, and click Go. If you choose the latter, you need only click Open Fasta  
 +
and choose your FASTA file to open. Two solutions are provided on the right</span><span>——</span><span lang="EN-US">one
 +
is solved by Tom Tuschl’s method and the other is by rational design. <o:p></o:p>
 +
</span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<h1><a name="_Toc336260394"><span lang="EN-US">MetaNetwork</span></a><span lang="EN-US"><o:p></o:p></span></h1>
 +
<p><o:wrapblock>
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">
 +
  <v:imagedata src="file:///I:\msohtmlclip1\01\clip_image011.png" o:title=""/>
 +
  <o:lock v:ext="edit" aspectratio="f"/>
 +
  <w:wrap type="topAndBottom" xmlns:w="urn:schemas-microsoft-com:office:word"/>
 +
</v:shape></o:wrapblock><br clear="ALL">
 +
<span lang="EN-US">Procedure:<span> </span><o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/7/79/Image011.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/6/6f/Image0012.jpg" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_13"
 +
o:spid="_x0000_i1040" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image012.jpg" o:title=""/>
 +
</v:shape>In the main display window, you will see a network from KEGG website.  
 +
On the left box displays a list of the files you have opened. Pay attention that  
 +
you can right click the main display window to open another KEGG xml file or to  
 +
search any element in the network (choose find element).<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<h1><a name="_Toc336260395"><span lang="EN-US">Simulator</span></a><span lang="EN-US"><o:p></o:p></span></h1>
 +
<p><span lang="EN-US">Procedure: <span>
 +
<v:shape id="图示_x0020_3" o:spid="_x0000_i1039"
 +
type="#_x0000_t75"
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">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image013.png" o:title=""/>
 +
<o:lock v:ext="edit" aspectratio="f"/>
 +
</v:shape><v:shape id="图片_x0020_16" o:spid="_x0000_i1038" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image014.jpg" o:title=""/>
 +
</v:shape></span><o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/9/9e/Image013.png" style="width: 650px;></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/0/07/Image014.jpg"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/5/5b/Image015.png" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_15"
 +
o:spid="_x0000_i1037" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image015.png" o:title=""/>
 +
</v:shape>There are 5 options for you to select: exhaustive single deletion,  
 +
reaction deletion, Fva analysis, <span>Perturbation analysis</span> and <span>
 +
PhPP analysis</span>. They with FBA solve will be introduced in detail. You can  
 +
select all of them or part of them, or even nothing, then click Go. If you  
 +
select none of them, you will see a table showed basic information of each  
 +
reaction. If you select several of them, the corresponding windows will tiled in  
 +
the resulting window. If you select reaction deletion option, a selection of the  
 +
reaction you want to wipe off is required, and if there is any change, it will  
 +
be highlighted in red font.<o:p></o:p></span></p>
 +
<p><span lang="EN-US">1.FBA_solve<span><v:shape id="图片_x0020_28"
 +
o:spid="_x0000_i1036" type="#_x0000_t75" alt="说明: fba_sove.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image016.png" o:title="fba_sove"/>
 +
</v:shape></span><o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/b/ba/Image016.png" style="width: 650px;></p>
 +
<p><span lang="EN-US">Left column displays names of the reactions while the
 +
right one displays the flux amount of each reaction<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US">2. exanstive single deletion<o:p></o:p></span></p>
 +
<p><span lang="EN-US">Calculate the flux amount of the objective reaction when
 +
each other reaction is deleted one by one.<o:p></o:p></span></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_27"
 +
o:spid="_x0000_i1035" type="#_x0000_t75" alt="说明: exaustive single deletion.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image017.png" o:title="exaustive single deletion"/>
 +
</v:shape><o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span><img src="https://static.igem.org/mediawiki/igem.org/b/b2/Image017.png" style="width: 650px;></p>
 +
<p><span lang="EN-US">Left column display names of the reactions <o:p></o:p>
 +
</span></p>
 +
<p><span lang="EN-US">The right one displays the flux amount of objective
 +
reaction the user has chosen, when each other reaction is deleted one by one.<o:p></o:p></span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/d/d3/Image018.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/d/dc/Image019.png"></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span><img src="https://static.igem.org/mediawiki/igem.org/7/71/Image020.png"></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_26"
 +
o:spid="_x0000_i1034" type="#_x0000_t75" alt="说明: lethal deletions.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image018.png" o:title="lethal deletions"/>
 +
</v:shape>.<span><v:shape
 +
id="图片_x0020_25" o:spid="_x0000_i1033" type="#_x0000_t75" alt="说明: sub optimal dels.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image019.png" o:title="sub optimal dels"/>
 +
</v:shape></span>when rolling down the screen, you can see the
 +
reaction-names related to lethal deletions, sub optimal deletions and super
 +
optimaldeletions<span>.<o:p></o:p></span></span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/f/f1/Image021.png"></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_23"
 +
o:spid="_x0000_i1031" type="#_x0000_t75" alt="说明: reaction subsystem">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image021.png" o:title="reaction subsystem"
 +
  croptop="3988f" cropbottom="3190f"/>
 +
</v:shape><o:p></o:p></span></p>
 +
<p><span lang="EN-US">This graph shows how many lethal knockouts in each
 +
reaction subsystem. The information of the reaction subsystem can be attained in
 +
SBML <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/2/28/Image022.png"></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_22"
 +
o:spid="_x0000_i1030" type="#_x0000_t75" alt="说明: single%20knock">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image022.png" o:title="single%20knock"
 +
  croptop="1456f" cropbottom="3311f"/>
 +
</v:shape><o:p></o:p></span></p>
 +
<p><span lang="EN-US">This hist graph shows the frequency of reaction which has
 +
different quantified effect showed in the X-axis opon objective reaction.<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US">3. reaction deletion<o:p></o:p></span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/8/81/Image023.png" style="width: 650px;></p>
 +
<p>
 +
<v:shape id="图片_x0020_21"
 +
o:spid="_x0000_i1029" type="#_x0000_t75" alt="说明: reacion deletions.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image023.png" o:title="reacion deletions"/>
 +
</v:shape><span lang="EN-US">Middle column displays the flux amount of the wild type
 +
while the right column displays the mutant type, which is calculated based on
 +
the deletion of one chosen reaction.<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US">4. FVA analysis<o:p></o:p></span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/0/0d/Image024.png" style="width: 650px;></p>
 +
<p>
 +
<v:shape id="图片_x0020_20"
 +
o:spid="_x0000_i1028" type="#_x0000_t75" alt="说明: fva.bmp">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image024.png" o:title="fva"/>
 +
</v:shape><span lang="EN-US">This analysis exams the robustness of the whole metabolic
 +
network by calculating flux value which can be reached in reality. In other
 +
words, this function works out the upper and lower limit of the flux of each
 +
reaction, as you can see in the column name </span><span>‘</span><span lang="EN-US">lower
 +
flux limit </span><span>’</span><span lang="EN-US">and </span><span>‘</span><span lang="EN-US">upper
 +
flux limit</span><span>’</span><span lang="EN-US">.<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US">5.Perturbation analysis:</span></p>
 +
<p><img src="https://static.igem.org/mediawiki/igem.org/0/05/Image025.png"></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_11"
 +
o:spid="_x0000_i1027" type="#_x0000_t75" alt="说明: perturbation">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image025.png" o:title="perturbation"/>
 +
</v:shape><o:p></o:p></span></p>
 +
<p><span lang="EN-US">X-axis refers to the continuously increasing flux value of
 +
the chosen reaction.<o:p></o:p></span></p>
 +
<p><span lang="EN-US">Y-axis refers to the flux value of the objective reaction
 +
according to the variation of the flux value of chosen reaction in the X-axis<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US">6.PhPP analysis <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p><img src="https://static.igem.org/mediawiki/igem.org/8/8d/Image026.png"></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_10"
 +
o:spid="_x0000_i1026" type="#_x0000_t75" alt="说明: PHPP">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image026.png" o:title="PHPP"/>
 +
</v:shape><o:p></o:p></span></p>
 +
<p><span lang="EN-US">The value marked on the contour represents the flux value
 +
of the objective reaction based on the variation of two chosen reactions The
 +
color of the graph changes according to the altitude, namely, the objective flux
 +
value.</span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
 +
<h1><a name="_Toc336260396"><span lang="EN-US">G-Circle</span></a><span lang="EN-US"><o:p></o:p></span></h1>
 +
<p><o:wrapblock>
 +
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  <v:imagedata src="file:///I:\msohtmlclip1\01\clip_image027.png" o:title=""
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  croptop="-3052f"/>
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  <o:lock v:ext="edit" aspectratio="f"/>
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  <w:wrap type="topAndBottom" xmlns:w="urn:schemas-microsoft-com:office:word"/>
 +
</v:shape></o:wrapblock><br clear="ALL">
 +
<span lang="EN-US">Procedure: <o:p></o:p>
 +
</span></p>
 +
<p>
 +
<o:p></o:p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/9/94/Image027.png"></p>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/igem.org/9/98/Image028.jpg" style="width: 650px;></p>
 +
<p><span lang="EN-US">
 +
<v:shape id="图片_x0020_18"
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o:spid="_x0000_i1025" type="#_x0000_t75">
 +
<v:imagedata src="file:///I:\msohtmlclip1\01\clip_image028.jpg" o:title=""/>
 +
</v:shape>You can open several file so that you can compare the difference of
 +
these genomes. Each genome have 2 circles in this picture: the outer one is the
 +
genes of the genome, the inner is the expression level of corresponding genes
 +
under two different conditions. By the way, right click different sites in the
 +
small panoramic window or left click and drag the picture in the zoom window,
 +
you can move to anywhere you want to enlarge and see more details in the zoom
 +
window.<o:p></o:p></span></p>
 +
<p><span lang="EN-US"><o:p>&nbsp;</o:p></span></p>
</div>
</div>
               </div><!--end entry-->
               </div><!--end entry-->

Revision as of 12:14, 26 September 2012

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Our Project

Contents

Genome Browser


Procedure:


In the main display window, the first ribbon is the sequence that the user has inputted, and the third ribbon shows the corresponding gene annotation. You can click any annotation that you want, and then the sequence site will automatically jump to the fragment related to it. Between those ribbons is an adjustable slider, which can adjust your view location by sliding to the left or to the right. Above the sequence are the head and tail of the location that you are viewing while the head and tail of the whole sequence are displayed in the bottom textbox. What’s more, the textbox contains the length, product, tag of the sequence, and links of related papers are also included sometimes.

On the left, there are two useful tools: you can search the fragment that you want by position, product or name (Position represents the position of its head, Product and Name stand for products and names of related genes, both of them support fuzzy search), besides, whether you choose product or name, a dialog box that contains names of related genes will appear; the slider of Zoom is designed for regulating the display frame rate of the sequence.

Biobrick


Procedure:

In the main display window, you can view the information of existing biobricks, which are all localized and enable you to get rid of loading when you are studying a biobrick. On the left is the list of the files that you have opened.

 

Riboswitch

Procedure:

On the right is the main operating area and you can choose your target type (your experimental object) and corresponding target name, aptamer and regulation direction. Attention should be paid to the input box named Input your Target. You can search your target name by key words or even several letters of it. It should be useful to you. Click GO and you will see a picture. The designed riboswitch is in the dotted box on the top left corner. Below it is a diagram to show how the riboswitch works. Besides, there is a list on the left box. It records your choices that you have made in created riboswich project, and you need only click GO next time.

 

SiRNA


Procedure:

The left textbox is for you to input DNA sequence. There are two ways to input that: you can either input the sequence directly or open a file in FASTA format. If you choose the former, click Direct Input, then input your sequence, and click Go. If you choose the latter, you need only click Open Fasta and choose your FASTA file to open. Two solutions are provided on the right——one is solved by Tom Tuschl’s method and the other is by rational design.

 

MetaNetwork


Procedure:

In the main display window, you will see a network from KEGG website. On the left box displays a list of the files you have opened. Pay attention that you can right click the main display window to open another KEGG xml file or to search any element in the network (choose find element).

 

Simulator

Procedure:

There are 5 options for you to select: exhaustive single deletion, reaction deletion, Fva analysis, Perturbation analysis and PhPP analysis. They with FBA solve will be introduced in detail. You can select all of them or part of them, or even nothing, then click Go. If you select none of them, you will see a table showed basic information of each reaction. If you select several of them, the corresponding windows will tiled in the resulting window. If you select reaction deletion option, a selection of the reaction you want to wipe off is required, and if there is any change, it will be highlighted in red font.

1.FBA_solve

Left column displays names of the reactions while the right one displays the flux amount of each reaction

 

2. exanstive single deletion

Calculate the flux amount of the objective reaction when each other reaction is deleted one by one.

 Left column display names of the reactions

The right one displays the flux amount of objective reaction the user has chosen, when each other reaction is deleted one by one.

 

. when rolling down the screen, you can see the reaction-names related to lethal deletions, sub optimal deletions and super optimaldeletions.

This graph shows how many lethal knockouts in each reaction subsystem. The information of the reaction subsystem can be attained in SBML

This hist graph shows the frequency of reaction which has different quantified effect showed in the X-axis opon objective reaction.

 

3. reaction deletion

说明: reacion deletions.bmp Middle column displays the flux amount of the wild type while the right column displays the mutant type, which is calculated based on the deletion of one chosen reaction.

 

4. FVA analysis

说明: fva.bmp This analysis exams the robustness of the whole metabolic network by calculating flux value which can be reached in reality. In other words, this function works out the upper and lower limit of the flux of each reaction, as you can see in the column name ‘lower flux limit ’and ‘upper flux limit’.

 

5.Perturbation analysis:

X-axis refers to the continuously increasing flux value of the chosen reaction.

Y-axis refers to the flux value of the objective reaction according to the variation of the flux value of chosen reaction in the X-axis

 

 

6.PhPP analysis

The value marked on the contour represents the flux value of the objective reaction based on the variation of two chosen reactions The color of the graph changes according to the altitude, namely, the objective flux value.

 

 

G-Circle


Procedure:

You can open several file so that you can compare the difference of these genomes. Each genome have 2 circles in this picture: the outer one is the genes of the genome, the inner is the expression level of corresponding genes under two different conditions. By the way, right click different sites in the small panoramic window or left click and drag the picture in the zoom window, you can move to anywhere you want to enlarge and see more details in the zoom window.

 

Retrieved from "http://2012.igem.org/Team:SYSU-Software/Tutorial"