Team:Freiburg

From 2012.igem.org

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Revision as of 21:24, 25 September 2012

Welcome to the FreiGEM 2012 wiki,

on this wiki, we want to present our project for the iGEM 2012 competition and introduce the freiGEM 2012 team.

Let us tell you a fabulous TALE ...
Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from Xanthomonas spp., this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world.

Stay tuned to FreiGEM 2012 - we will keep you in the loop.

The FreiGEM 2012 Team

With kind support of

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 <div align="justify">'''Welcome to the FreiGEM 2012 wiki''',
-in this wiki we want to present our project for the iGEM 2012 contest and introduce the freigem 2012 team.
+on this wiki, we want to present our project for the iGEM 2012 competition and introduce the freiGEM 2012 team.
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-The iGEM Team Freiburg 2012 (FreiGEM) is dedicated to standardizing the synthesis of Transactivator-like (TAL) proteins, i.e. DNA-binding proteins. In contrast to zinc-finger proteins they consist of domain-like repeats in their primary structure that differ only by two amino acids. These two amino acids of a given repeat determine which DNA base the repeat will bind to. By providing efficient mechanisms to combine the different repeats in order to target a certain DNA sequence of around 14bp the FreiGEM team is working towards establishing a standardized kit. This one tube essay will provide the user with an easy, quick and cost-efficient way to design his or her individualized TAL protein in just a glance.
+'''Let us tell you a fabulous TALE ...'''<br>
+Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from Xanthomonas spp., this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world.