Team:SDU-Denmark/labwork/Protocols/Checkdigest

From 2012.igem.org

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<h1> Check Digest </h1>
<h1> Check Digest </h1>
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<h2> </h2> </b>
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<p>
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<h2>Digest mix, using Fastdigest enzymes</h2>
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</br></br>
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Digestion Mix
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<pre>
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<pre>
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4 µL H2O
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0,5 µL enzyme A
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0,5 µL enzyme B
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2 µL Fast Digest green buffer
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3 µL dna product
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</pre>
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</pre>
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</br>
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<p>
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</br>
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Digest</br>
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1)Cast an agarose gel for purification of the cut product</br></br>
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2)Mix the restriction mixture in an eppendorf tube</br></br>
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3)Leave for 5 min. at 37°C (no shaking!)</br></br>
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4)Immidiately load the restriction mixture in the gel</br></br>
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5)Run the gel, cut out and purify bands of correct size.</br></br>

Revision as of 00:01, 25 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID





mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Colony-PCR Transformation Gel-electrophoresis
Reverse Transcriptase Mutagenisis PCR-,gel clean-up Content

Check Digest

Digest mix, using Fastdigest enzymes



Digestion Mix 
4 µL H2O 
0,5 µL enzyme A
0,5 µL enzyme B
2 µL Fast Digest green buffer
3 µL dna product


Digest
1)Cast an agarose gel for purification of the cut product

2)Mix the restriction mixture in an eppendorf tube

3)Leave for 5 min. at 37°C (no shaking!)

4)Immidiately load the restriction mixture in the gel

5)Run the gel, cut out and purify bands of correct size.

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