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Team:UC Chile/Cyano/Labook/april
From 2012.igem.org
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[[File:aprillabook_uc_chile.jpg|center]] | [[File:aprillabook_uc_chile.jpg|center]] | ||
| - | <font size="4">Monthly | + | <font size="4">Monthly Summary</font> |
<br><br> | <br><br> | ||
PCR runs have been defective so we haven’t been able to obtain all the parts needed to assemble our constructs. Nonetheless, parts for C1.1 and C2.1 amplified and a Gibson assembly was attempted to join them. The assembly was incorrect according to a restriction enzyme digestion protocol. | PCR runs have been defective so we haven’t been able to obtain all the parts needed to assemble our constructs. Nonetheless, parts for C1.1 and C2.1 amplified and a Gibson assembly was attempted to join them. The assembly was incorrect according to a restriction enzyme digestion protocol. | ||
Synechocytis are growing ok. | Synechocytis are growing ok. | ||
| + | |||
| + | <font size="4">Monthly Summary</font> | ||
| + | <br><br> | ||
| + | Attempted synechocytis DNA extraction according to protocol. There was no pellet and no DNA. | ||
| + | |||
| + | Synechocystis cultures were observed under acridine dye and turned out to be axenic. (upload photos). Cultures were reinoculated in fresh BG11. | ||
| + | |||
| + | Started a growth curve for Synechocystis (that was never finished). | ||
| + | |||
Revision as of 21:16, 23 September 2012
April
Monthly Summary
PCR runs have been defective so we haven’t been able to obtain all the parts needed to assemble our constructs. Nonetheless, parts for C1.1 and C2.1 amplified and a Gibson assembly was attempted to join them. The assembly was incorrect according to a restriction enzyme digestion protocol.
Synechocytis are growing ok.
Monthly Summary
Attempted synechocytis DNA extraction according to protocol. There was no pellet and no DNA.
Synechocystis cultures were observed under acridine dye and turned out to be axenic. (upload photos). Cultures were reinoculated in fresh BG11.
Started a growth curve for Synechocystis (that was never finished).
