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Team:UC Chile/Labook/march
From 2012.igem.org
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Amplification of parts from 2011 kit: P1003, B0014 and others (specify) are needed for constructs. | Amplification of parts from 2011 kit: P1003, B0014 and others (specify) are needed for constructs. | ||
Standard Biobrick assembly. | Standard Biobrick assembly. | ||
| - | Transformation | + | Transformation. |
| - | Plated, only one surviving colony | + | Plated, only one surviving colony. |
Conclusion: psB1C3 was already CUT | Conclusion: psB1C3 was already CUT | ||
PCR’s. By electrophoresis we have: | PCR’s. By electrophoresis we have: | ||
Revision as of 20:59, 23 September 2012
March
Constructs and primers design
First constructs (link to constructs)
Primer sequences
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Strategy for integration construct: Part K292003 is not in the kit. It will be assembled from P1003+B0012+B0014
Getting parts ready
Plates prepared Broad host plasmid transformation Batch of new competent bacteria ready Primer alicuoting (primers asked for in late January).
Amplification of parts from 2011 kit: P1003, B0014 and others (specify) are needed for constructs. Standard Biobrick assembly.
Transformation.
Plated, only one surviving colony. Conclusion: psB1C3 was already CUT
PCR’s. By electrophoresis we have:
Unsuccessful attempts: psbAB,psbAB2, Kanr, RS1. Success: RFP, RS2, backbone. Comment: seems as primers for expression plasmid are problematic. Kanr new PCR attempts. Unsuccessful results. PCR for pPMQAK1, psbAB, RFP.
Work plan
Labour division: Bactomithril {Max, Emilia,Ulises, Claudia, Bryon}
Cyano {Simón, Carla, Tamara, Isaac, Sebastián}
Meetings with Dr. Gutiérrez every two weeks to check work done.
